拟黑多刺蚁活性组分对系统性红斑狼疮的治疗作用及机制

毛长智; 何俊慧; 李冬梅; 何飞; 陆国寿; 胡筱希; 韦桂宁

doi:10.13422/j.cnki.syfjx.20191703

您当前的位置：

首页 >

文章列表页 >

拟黑多刺蚁活性组分对系统性红斑狼疮的治疗作用及机制

药理 | 更新时间：2021-02-09

- 拟黑多刺蚁活性组分对系统性红斑狼疮的治疗作用及机制
- Effect and Mechanism of Active Fraction of Polyrhachis vicina in Treatment of Systemic Lupus Erythematosus(SLE)
- 中国实验方剂学杂志 2019年25卷第18期页码：65-70
- 作者机构：
  
  1.南宁市第三人民医院，南宁　 530003；
  2.广西壮族自治区中医药研究院，南宁　 530022
- 作者简介：
  
  毛长智，副主任药师，从事医院药学研究，E-mail: 339581018@qq.com
  韦桂宁，硕士，主任药师，从事中药药理毒理研究，Tel: 0771-5869102，E-mail：weiguining2004@163.com
- 基金信息：
  
  国家自然科学基金项目(81360653;81560663;81703769);广西科技计划项目(2018GXNSFDA281046;桂科AD18216002;2011GXNSFA018258)
- DOI：10.13422/j.cnki.syfjx.20191703
  中图分类号： R2-0; R22; R285.5; R289
- 收稿日期：2019-02-08，
  
  网络出版日期：2019-05-16，
  
  纸质出版日期：2019-09-20
- 稿件说明：
移动端阅览
毛长智, 何俊慧, 李冬梅, 等. 拟黑多刺蚁活性组分对系统性红斑狼疮的治疗作用及机制[J]. 中国实验方剂学杂志, 2019,25(18):65-70.

Zhang-zhi MAO, Jun-hui HE, Dong-mei LI, et al. Effect and Mechanism of Active Fraction of Polyrhachis vicina in Treatment of Systemic Lupus Erythematosus(SLE)[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 65-70.
毛长智, 何俊慧, 李冬梅, 等. 拟黑多刺蚁活性组分对系统性红斑狼疮的治疗作用及机制[J]. 中国实验方剂学杂志, 2019,25(18):65-70. DOI： 10.13422/j.cnki.syfjx.20191703.

Zhang-zhi MAO, Jun-hui HE, Dong-mei LI, et al. Effect and Mechanism of Active Fraction of Polyrhachis vicina in Treatment of Systemic Lupus Erythematosus(SLE)[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(18): 65-70. DOI： 10.13422/j.cnki.syfjx.20191703.

摘要

目的：

采用动物模型和细胞模型，探讨拟黑多刺蚁活性组分(AFPV)对系统性红斑狼疮(SLE)的作用及可能机制。

方法：

采用大鼠Arthus反应，将60只SD大鼠随机分为正常组，模型组，醋酸泼尼松组(5 mg·kg

－1

)，AFPV高、中、低剂量组(400，200，100 mg·kg

－1

)。采用牛血清蛋白-弗氏完全(不完全)佐剂复制SLE模型。观察AFPV对SLE大鼠背部皮肤红肿直径的影响，酶联免疫吸附测定检测血清抗双链DNA(dsDNA)抗体，补体3(C3)，补体4(C4)，免疫球蛋白M(IgM)，白细胞介素-1(IL-1)，白细胞介素-6(IL-6)，白细胞介素-31(IL-31)，白细胞介素-33(IL-33)水平；免疫磁珠法分选16～18周MRL/lpr狼疮小鼠和C57BL/6J正常小鼠脾脏CD4

＋

T细胞，观察AFPV对miR-200a，miR-155的表达及沉默E盒锌指结合蛋白1(ZEB1)和细胞因子信号转导抑制因子1(SOCS1)水平的影响，探讨AFPV对SLE的作用及可能机制。

结果：

与正常组比较，模型组大鼠背部皮肤红肿直径显著增大(

0.01)，与模型组比较，AFPV高、中剂量组可显著降低SLE大鼠背部皮肤红肿直径(

0.05，

0.01)；与正常组比较，模型组大鼠血清IgM，IL-6，IL-33明显增高(

0.05)，与模型组大鼠比较，AFPV高、中给药组IgM，IL-6，IL-33明显降低(

0.05)；与正常组比较，MRL/lpr狼疮小鼠的CD4

＋

T细胞miR-200a的表达显著降低，miR-155的表达显著增高及ZEB1水平显著增高而SOCS1的表达显著降低(

0.01)，与模型组比较，AFPV干预后miR-200a的表达显著增高，miR-155的表达显著降低(

0.01)，ZEB1水平显著降低而SOCS1的表达显著增高(

0.01)。

结论：

AFPV对SLE大鼠具有一定的治疗作用，其机制可能与其对miR-200a，miR-155及ZEB1 和SOCS1的调节有关。

Abstract

Objective:

To observe the effect of an active fraction of

Polyrhachis vicina

(AFPV) on systemic lupus erythematosus (SLE) and its possible mechanism based on animal and cell models.

Method:

Totally 60 SD rats were randomly divided into normal control group

model group

prednisone acetate group (5 mg·kg

-1

)

and high

medium and low-dose AFPV groups (400

200

100 mg·kg

-1

). SLE model was replicated with bovine serum albumin-Freund's complete (incomplete) adjuvant. Arthus reaction was observed to study the effect of AFPV on the diameter of back skin redness in rats with SLE. The expressions of anti-double-stranded DNA (dsDNA) antibody

complements 3 (C3)

complement 4 (C4)

immunoglobulin M (IgM)

interleukin-1 (IL-1)

interleukin-6 (IL-6)

interleukin-31 (IL-31) and interleukin-33 (IL-33) in serum were detected by enzyme-linked immunosorbent assay. CD4

T cells were isolated from the spleens of MRL/lpr and C57BL/6J mice at the age of 16 to 18 weeks by immunomagnetic beads method. The expressions of miR-200a and miR-155 and the levels of zinc-finger-enhancer binding protein 1(ZEB1) and suppressor of cytokine signaling1(SOCS1) in CD4

T cells were observed to explore the effect of AFPV on SLE and its possible mechanism.

Result:

Compared with the normal group

the diameter of back skin swelling in the model group was significantly increased (

0.01). Compared with the model group

the high and medium-dose AFPV groups significantly reduce the skin redness on the back of SLE rats (

0.05

0.01); Compared with the normal group

the serum levels of IgM

IL-6 and IL-33 were significantly increased in the model group (

0.05). Compared with the model group

the serum levels of IgM

IL-6 and IL-33 were significantly decreased after the intervention of AFPV (

0.05). Compared with the normal group

the expression of miR-200a was significantly decreased (

0.01)

and its target protein ZEB1 was significantly increased (

0.01) in the CD4

T cells of MRL/lpr lupus mice. Compared with the model group

the expression of microRNA-200a increased significantly

the expression of microRNA-155 decreased significantly (

0.01)

the level of ZEB1 decreased significantly

and the expression of SOCS1 increased significantly after AFPV intervention(

0.01).

Conclusion:

AFPV has therapeutic effect on rats with SLE

its mechanism may be related to the regulation of miR-200a/ZEB1 and miR-155/SOCS1.

关键词

Keywords

references

Gergianaki I , Bortoluzzi A , Bertsias G . Update on the epidemiology, risk factors, and disease outcomes of systemic lupus erythematosus [J]. Best Pract Res Clin Rheumatol , 2018 , 32 ( 2 ): 188 - 205 .

El Qadiry R , Bourrahouat A , Aitsab I , et al . Systemic lupus erythematosus-related pancreatitis in children: severe and lethal form [J]. Case Rep Pediatr , 2018 , 9 : e4612754 .

Ali A , Sayyed Z , Ameer M A , et al . Systemic lupus erythematosus: an overview of the disease pathology and its management [J]. Cureus , 2018 , 10 ( 9 ): e3288 .

何飞，李冬梅，苏启表，等．拟黑多刺蚁活性组分治疗小鼠皮肤瘙痒的实验研究 [J]．中药材， 2018 ， 41 ( 5 )： 1201 - 1204 ．

韦桂宁，曾宪彪，牙启康，等．拟黑多翅蚁抗痛风作用研究 [J]．中国实验方剂学杂志， 2012 ， 18 ( 20 )： 205 - 208 ．

张欣，龙倩，楚世峰，等．拟黑多刺蚁石油醚部位对抑郁大鼠神经炎症反应的抑制作用 [J]．药学学报， 2018 ， 53 ( 7 )： 1042 - 1047 ．

SU Q , SU H , NONG Z , et al . Hypouricemic and nephroprotective effects of an active fraction from polyrhachis vicina roger on potassium oxonate-induced hyperuricemia in rats [J]. Kidney Blood Press Res , 2018 , 43 ( 1 ): 220 - 233 .

WEI G N , CHU S F , SU Q B , et al . Antidepressant-like effect of active fraction of Polyrhachisvicina Roger in a rat depression model [J]. J Tradit Chin Med , 2018 , 38 ( 1 ): 12 - 21 .

王璟，汪悦，夏卫军，等．解毒祛斑汤对大鼠Arthus反应的影响 [J]．中国中医急症， 2007 ， 16 ( 2 )： 204 - 205 ．

张萍，刘红云，刘晓燕，等．调节性T细胞在小鼠ITP发病中的作用 [J]．中国实验血液学杂志， 2016 ， 24 ( 3 )： 784 - 787 ．

罗建蓉，肖怀，张成桂，等．两种药用蜚蠊油脂成分的GC-MS分析 [J]．中国实验方剂学杂志， 2017 ， 23 ( 11 )： 55 - 58 ．

杜晨晖，闫艳，朱羽尧，等．山西不同产地酸枣仁中脂肪酸类成分的GC-MS分析 [J]．中国实验方剂学杂志， 2018 ， 24 ( 24 )： 19 - 25 ．

SU Q B , SU H , NONG Z H , et al . Hypouricemic and nephroprotective effects of an active fraction from Polyrhachis vicina Roger on potassium oxonate-induced hyperuricemia in rats [J]. Kidney Blood Press Res , 2018 , 43 ( 1 ): 220 - 233 .

曹文婷，孙萍，李华艳，等． n-3多不饱和脂肪酸在不同疾病中的研究进展 [J]．山西医药杂志， 2014 ， 43 ( 14 )： 1668 - 1670 ．

徐丽敏，陈学荣，屠呦呦．双氢青蒿素对红斑狼疮性BXSB小鼠的作用 [J]．中国中西医结合皮肤性病学杂志， 2002 ， 1 ( 1 )： 19 - 20 ．

MO Y M , FANG P H , WAI K I , et al . Serum levels of IL-33 and soluble ST2 and their association with disease activity in systemic lupus erythematosus [J]. Rheumatology (Oxford) , 2010 , 49 ( 3 ): 520 - 527 .

Düster M , Becker M , Gnirck A C , et al . T cell-derived IFN-downregulates protective group 2 innate lymphoid cells in murine lupus erythematosus [J]. Eur J Immunol , 2018 , 48 ( 8 ): 1364 - 1375 .

GUO J , XIANG Y , PENG Y F , et al . The association of novel IL-33 polymorphisms with sIL-33 and risk of systemic lupus erythematosus [J]. Mol Immunol , 2016 , 77 : 1 - 7 .

WAN A W , Mohd Shahrir M S , Asrul W , et al . The association between serum prolactin levels and interleukin-6 and systemic lupus erythematosus activity [J]. Reumatismo , 2018 , 70 ( 4 ): 241 - 250 .

Karampetsou M P , Comte D , Suárez-Fueyo A , et al . Signaling lymphocytic activation molecule family member 1 engagement inhibits T cell-B cell interaction and diminishes interleukin-6 production and plasmablast differentiation in systemic lupus erythematosus [J]. Arthritis Rheumatol , 2019 , 71 ( 1 ): 99 - 108 .

Lashine Y A , Salah S , Aboelenein H R , et al . Correcting the expression f miRNA-155 represses PP2AC and enhances the release of IL-2 in pbmcs of juvenile SLE patients [J]. Lupus , 2015 , 24 ( 3 ): 240 - 247 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据