Objective:

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To screen the specific reverse primers of Galli Gigerii Endothelium Corneum

duck gizzard membrane and goose gizzard membrane

and establish a specific PCR for molecular identifying Galli Gigerii Endothelium Corneum and its common adulterants.

Method:

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Based on the mutation sites on the 12S rRNA sequence

specific polymerase Chain reaction(PCR) identify primers were designed for chicken

duck and goose gizzard membrane. The specific PCR reaction conditions were optimized

and the PCR identification method was explored and verified in terms of tolerance and feasibility. Thirty batches of Galli Gigerii Endothelium Corneum decoction pieces extracted from the test were identified.

Result:

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Thirty batches of Galli Gigerii Endothelium Corneum decoction pieces were detected using chicken-specific primers

273 bp of specific bands was amplified and visualized on the agarose electrophoregram. When duck and goose primers were used

no corresponding amplified band was detected.

Conclusion:

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The allele-specific PCR method can be used as a rapid and accurate method to identify Galli Gigerii Endothelium Corneum. It is a promise method for special sampling tasks of Chinese herbal medicine and decoction tablets nationwide.