酸枣仁汤通过抑制APP/PS1双转基因小鼠海马神经炎症发挥神经保护作用

龙清华; 丁莉; 赵宾宾; 徐波; 谭爱华; 王平

doi:10.13422/j.cnki.syfjx.20191936

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酸枣仁汤通过抑制APP/PS1双转基因小鼠海马神经炎症发挥神经保护作用

经典名方 | 更新时间：2021-02-09

- 酸枣仁汤通过抑制APP/PS1双转基因小鼠海马神经炎症发挥神经保护作用
- Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice
- 中国实验方剂学杂志 2019年25卷第20期页码：1-6
- 作者机构：
  
  湖北中医药大学　老年医学研究所，武汉　 430065
- 作者简介：
  
  龙清华，在读博士，从事中医药防治老年病研究，E-mail：287201413@qq.com
  王平，教授，主任医师，博士生导师，从事中医衰老理论及老年病证治疗规律研究，E-mail：pwang54@aliyun.com
- 基金信息：
  
  国家自然科学基金项目(81573865;81703972)
- DOI：10.13422/j.cnki.syfjx.20191936
  中图分类号： R2-0; R285.5; R289
- 收稿日期：2018-04-25，
  
  网络出版日期：2019-06-19，
  
  纸质出版日期：2019-10-20
- 稿件说明：
移动端阅览
龙清华, 丁莉, 赵宾宾, 等. 酸枣仁汤通过抑制APP/PS1双转基因小鼠海马神经炎症发挥神经保护作用[J]. 中国实验方剂学杂志, 2019,25(20):1-6.

Qing-hua LONG, Li DING, Bin-bin ZHAO, et al. Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 1-6.
龙清华, 丁莉, 赵宾宾, 等. 酸枣仁汤通过抑制APP/PS1双转基因小鼠海马神经炎症发挥神经保护作用[J]. 中国实验方剂学杂志, 2019,25(20):1-6. DOI： 10.13422/j.cnki.syfjx.20191936.

Qing-hua LONG, Li DING, Bin-bin ZHAO, et al. Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 1-6. DOI： 10.13422/j.cnki.syfjx.20191936.

摘要

目的：

观察酸枣仁汤对APP/PS1小鼠海马神经炎症的影响，并探讨其神经保护的可能机制。

方法：

将小鼠随机分为空白组，模型组，多奈哌齐组(0.92 mg·kg

－1

)，酸枣仁汤低、高(12.96，25.92 g·kg

－1

)剂量组。各组连续给药30 d后，采用尼氏染色观察各组小鼠海马齿状回(DG)区病理形态的变化，采用酶联免疫吸附测定(ELISA)检测各组小鼠血清中肿瘤坏死因子-

(TNF-

)和白细胞介素-1

(IL-1

)表达水平，采用实时荧光定量聚合酶链式反应(Real-time PCR)检测各组小鼠海马中TNF-

，IL-1

的mRNA表达水平，采用免疫组化(IHC)和蛋白免疫印迹法(Western blot)检测各组小鼠海马中胶质纤维酸性蛋白(GFAP)和离子钙结合蛋白(IBA1)蛋白表达水平。

结果：

与空白组比较，模型组小鼠海马DG区颗粒细胞排列不均匀，细胞丢失明显，部分神经元胞内尼氏体消失或固缩，血清中TNF-

，IL-1

含量显著升高(

0.01)，小鼠海马中TNF-

，IL-1

mRNA表达显著升高(

0.01)，海马DG区中GFAP，IBA1蛋白表达显著上调(

0.01)；与模型组比较，多奈哌齐组、酸枣仁汤低、高剂量组小鼠海马DG区颗粒细胞排列稍整齐，神经元丢失减轻，且胞内尼氏体消失或固缩情况好转，小鼠血清中TNF-

，IL-1

含量明显降低(

0.05，

0.01)，小鼠海马中TNF-

，IL-1

mRNA表达明显降低(

0.05，

0.01)，小鼠海马DG区中GFAP，IBA1蛋白表达明显降低(

0.05，

0.01)。

结论：

酸枣仁汤能改善APP/PS1双转基因小鼠神经元丢失，其机制可能与其调节小鼠海马神经炎症有关。

Abstract

Objective:

To observe the effect of Suanzaoren Tang on hippocampal neuroinflammation in APP/PS1 mice and to explore its possible mechanism of neuroprotection.

Method:

The mice were randomly divided into blank group

model group

donepezil group(0.92 mg·kg

-1

)

Suanzaoren Tang low and high-dose groups(12.96

25.92 g·kg

-1

). After 30 days of continuous administration in each group

pathological changes of dentate gyrus (DG) in hippocampus of mice in each group were observed by Nissl staining.The levels of tumor necrosis factor-

(TNF-

) and interleukin-1

(IL-1

) in serum of each group were detected by enzyme-linked immunosorbent assay (ELISA). Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the mRNA expression levels of TNF-

and IL-1

in hippocampus of each group. The expression levels of glial fibrillary acidic protein (GFAP) and ionic calcium binding protein 1 (IBA1) in hippocampus of each group were detected by immunohistochemical staining (ICH) and Western blot.

Result:

Compared with blank group

the granule cells in DG region were unevenly arranged in model group

with obvious cell loss

and the nissl bodies in some neurons disappeared or condensed

serum TNF-

and IL-1

content significantly increased (

0.01)

mice hippocampus of TNF-

and IL-1

mRNA expression quantity significantly increased (

0.01)

levels of GFAP and IBA1 in the hippocampal DG region were significantly up-regulated (

0.01). Compared with model group

the granule cells in the hippocampal DG region were slightly aligned in donepezil group

Suanzaoren Tang low and high-dose groups

neuron loss of ease

and intracellular austenite disappear or pyknotic conditions improve

the expressions levels of TNF-

and IL-1

in the serum were decreased(

0.05

0.01)

the expression levels of TNF-

and IL-1

mRNA in the hippocampus were decreased(

0.05

0.01)

protein expression levels of GFAP and IBA1 in the hippocampal DG region were decreased(

0.05

0.01).

Conclusion:

Suanzaoren Tang can improve neuronal loss in APP/PS1 double transgenic mice

and its mechanism may be related to the regulation of hippocampal neuroinflammation in mice.

关键词

Keywords

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