霍山石斛的抗炎作用

汪蒙蒙; 季兆洁; 甘江华; 俞年军; 陈卫东; 彭代银; 韩岚

doi:10.13422/j.cnki.syfjx.20192004

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霍山石斛的抗炎作用

药理 | 更新时间：2021-02-09

- 霍山石斛的抗炎作用
- Anti-inflammatory Effect of Dendrobii Huoshanense Herba
- 中国实验方剂学杂志 2019年25卷第20期页码：76-81
- 作者机构：
  
  安徽中医药大学　药学院，中药复方安徽省重点实验室，安徽道地中药材品质提升协同创新中心，合肥　 230012
- 作者简介：
  
  汪蒙蒙，在读硕士，从事中药药理学研究，E-mail：1109129099@qq.com
  彭代银，教授，博士生导师，从事中药资源研究与开发、中药药理学研究，Tel：0551-65169007，Email：pengdaiyin@163.com；
  韩岚，博士，副教授，从事中药药理研究，E-mail：hanlan56@ahtcm.edu.cn
- 基金信息：
  
  安徽道地中药材品质提升协同创新中心项目(皖教科[2013]2号);安徽省教育厅项目(皖教秘科[2014]44号);安徽省科技重大专项计划项目(16030801104)
- DOI：10.13422/j.cnki.syfjx.20192004
  中图分类号： R2-0; R22; R285.5
- 收稿日期：2018-12-12，
  
  网络出版日期：2019-07-05，
  
  纸质出版日期：2019-10-20
- 稿件说明：
移动端阅览
汪蒙蒙, 季兆洁, 甘江华, 等. 霍山石斛的抗炎作用[J]. 中国实验方剂学杂志, 2019,25(20):76-81.

Meng-meng WANG, Zhao-jie JI, Jiang-hua GAN, et al. Anti-inflammatory Effect of Dendrobii Huoshanense Herba[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 76-81.
汪蒙蒙, 季兆洁, 甘江华, 等. 霍山石斛的抗炎作用[J]. 中国实验方剂学杂志, 2019,25(20):76-81. DOI： 10.13422/j.cnki.syfjx.20192004.

Meng-meng WANG, Zhao-jie JI, Jiang-hua GAN, et al. Anti-inflammatory Effect of Dendrobii Huoshanense Herba[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(20): 76-81. DOI： 10.13422/j.cnki.syfjx.20192004.

摘要

目的：

观察霍山石斛的抗炎作用。

方法：

60只昆明种小鼠，随机分为正常组、模型组、石斛高、中、低剂量组(4，2，1 g·kg

－1

)，醋酸地塞米松组(醋酸地塞米松，0.01 g·kg

－1

)；连续灌胃给药14 d；末次给药1 h后，于小鼠右耳的耳中部两侧滴二甲苯20 μL建立耳肿胀模型。1 h后断头处死所有小鼠，计算各组小鼠耳肿胀率。健康SD大鼠36只，分别为正常组、模型组、霍山石斛高、中、低(2.8，1.4，0.7 g·kg

－1

)剂量组、醋酸地塞米松组。连续灌胃给药7 d。末次灌胃给药1 h后，于各组大鼠的右后肢足趾皮下注射10%新鲜鸡蛋清建立足肿胀模型。分别于0，0.5，1，2，3，4，5 h时，测量各大鼠的右后足周长，计算大鼠的足肿胀度。取SD大鼠60只，向双侧腹股沟皮下植入无菌干燥棉球，分组同上。第2天灌胃给药，连续14 d。末次给药后，取出棉球，计算肉芽肿抑制率；检测各组大鼠血清中肿瘤坏死因子-

(TNF-

)，白细胞介素-1

(IL-1

)，白细胞介素-2(IL-2)，白细胞介素-6(IL-6)，

-干扰素(IFN-

)含量。

结果：

与正常组比较，模型组耳肿胀率、足肿胀度显著升高(

0.01)；与模型组比较，霍山石斛高、中、低剂量组均可明显降低小鼠耳肿胀率(

0.01)；霍山石斛高、中剂量组足肿胀度明显降低(

0.05，

0.01)；给药各组棉球肉芽干重显著下降(

0.01)，胸腺、脾脏指数没有显著性变化；与正常组比较，模型组大鼠血清中炎症因子含量显著升高(

0.01)；与模型组比较，霍山石斛高、中、低剂量组和醋酸地塞米松组炎症因子含量显著降低(

0.05，

0.01)。

结论：

霍山石斛能够有效抑制急、慢性炎症反应。

Abstract

Objective:

To observe the anti-inflammatory effect of Dendrobii Huoshanense Herba.

Method:

Totally 60 Kunming mice were randomly divided into normal group

model group

high

middle and low-dose Dendrobii Huoshanense Herba groups (DHS-H

DHS-M

DHS-L

1 g·kg

-1

) and dexamethasone acetate group (DXMS

0.01 g·kg

-1

). The rats were intragastrically administered for 14 days. After the last administration for 1 h

a total of 20 μL of xylene was added to both sides of the right ear center of the mice to establish the ear swelling model. All of the mice were decapitated 1 h later

and the ear swelling inhibition rate of each group were calculated. Totally 36 healthy SD rats were divided into normal model

model group

DHS-H

DHS-M

DHS-L groups (2.8

1.4

0.7 g·kg

-1

) and DXMS acetate group. The rats were intragastrically administered for 7 days. One hour after the last intragastric administration

a foot swelling model was established through subcutaneous injection of 10%fresh egg white in the right hind limb toe of each group. The right hind paw circumference of each rat was measured at 0

0.5

5 h

and the paw swelling of the rats was calculated. Totally 60 SD rats were implanted subcutaneously with sterile dry cotton balls in the bilateral groin and grouped as above. All of the rats were intragastrically administered for 14 days since the next day. After the last administration

cotton balls were taken out

and the inhibition rate of granuloma was calculated. And the contents of tumor necrosis factor-

(TNF-

)

interleukin-1

(IL-1

)

interleukin-2 (IL-2)

interleukin-6 (IL-6)

interferon-

(IFN-

) in the serum of each group were detected.

Result:

Compared with the normal group

the ear swelling rate

the foot swelling degree were significantly increased in the model group(

0.01). Compared with the model group

the swelling rate of mouse ears in high

middle and low-dose Dendrobii Huoshanense Herba groups reduced significantly (

0.01). The swelling degrees of the high and middle-dose groups were significantly decreased (

0.05

0.01). The dry weight of cotton buds in each group was significantly decreased (

0.01)

with no significant change in thymus and spleen index. Compared with the normal group

the serum levels of TNF-

IL-1

IL-2

IL-6

and IFN-

in the model group were higher than those in the normal group (

0.01). And compared with model group

the levels of TNF-

IL-1

IL-2

IL-6

and IFN-

in Dendrobii Huoshanense Herba group and positive drug group were decreased significantly (

0.05

0.01).

Conclusion:

Dendrobii Huoshanense Herba could effectively inhibit acute and chronic inflammatory reactions.

关键词

Keywords

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