Objective:

2

To establish the method for determining the content of Polygalae Radix (3

6′-disinapoylsucrose) and Glycyrrhizae Radix et Rhizoma (glycyrrhizic acid) in Fufang Jiegeng Zhike Pian

so as to know the overall quality condition of Fufang Jiegeng Zhike Pian and ensure the safety and effectiveness of public medication.

Method:

2

HPLC was performed of MG C

18

chromatographic column (4.6 mm×250 mm

5 μm) and mobile phase acetonitrile-0.05%phosphoric acid solution were used to test Polygalae Radix (3

6′-disinapoylsucrose) and Glycyrrhizae Radix et Rhizoma (glycyrrhizic acid)

with the sample amount of 20 μL

flow rate of 1.0 mL·min

-1

and column temperature of 30 ℃

where the mobile phase acetonitrile-0.05%phosphoric acid solution and detection wavelength were 18∶82 and 320 nm for Polygalae Radix (3

6′-disinapoylsucrose) and 35∶65 and 250 nm for Glycyrrhizae Radix et Rhizoma (glycyrrhizic acid).

Result:

2

3

6′-Disinapoylsucrose and glycyrrhizic acid presented high linearity with the peak area at 0.105 8-2.643 8 μg(

r

=0.999 5) and 0.077 0-5.773 0 μg (

r

=1.000 0) respectively

and the average recovery rate was 96.2%(RSD 1.2%) and 95.6%(RSD 0.9%). After content determination of Polygalae Radix and Glycyrrhizae Radix et Rhizoma in 178 batches of samples

it was found that some producers input no

only a few or even poor medicinal materials (medicinal slices) during production

and the quality varied a lot among products from different producers and products of different batches from a same producer.

Conclusion:

2

The method is simple

accurate

and highly reproducible and sensitive

providing scientific basis for the overall quality control of Fufang Jiegeng Zhike Pian.