芍药甘草汤主要成分在正常及多囊卵巢综合征大鼠尿液和粪便中的代谢产物分析

郭一婷; 程瑶; 邵云云; 刘俊瑾; 冯小娟; 张京平; 侯锐刚

doi:10.13422/j.cnki.syfjx.20192156

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芍药甘草汤主要成分在正常及多囊卵巢综合征大鼠尿液和粪便中的代谢产物分析

药物代谢 | 更新时间：2021-02-09

- 芍药甘草汤主要成分在正常及多囊卵巢综合征大鼠尿液和粪便中的代谢产物分析
- Analysis on Metabolites of Major Components of Shaoyao Gancaotang in Urine and Feces of Normal and Polycystic Ovary Syndrome Rats
- 中国实验方剂学杂志 2020年26卷第6期页码：103-112
- 作者机构：
  
  1.山西医科大学，太原　 030000
  2.山西医科大学　第二医院，太原　 030001
- 作者简介：
  
  [第一作者]　郭一婷，在读硕士，从事药效学及药代动力学研究，E-mail：Guoyit@126.com
  *侯锐刚，主任药师，从事药效学及药代动力学研究，Tel：0351-3365405，E-mail：houruigang9966@163.com
- 基金信息：
  
  山西省中药现代化关键技术研究振东专项(2014ZD0102);山西省卫生计生委科研课题(2015046)
- DOI：10.13422/j.cnki.syfjx.20192156
  中图分类号： R22; R969.1; R28; O657.6; R574.4
- 收稿日期：2019-05-08，
  
  网络出版日期：2019-07-22，
  
  纸质出版日期：2020-03-20
- 稿件说明：
移动端阅览
郭一婷, 程瑶, 邵云云, 等. 芍药甘草汤主要成分在正常及多囊卵巢综合征大鼠尿液和粪便中的代谢产物分析[J]. 中国实验方剂学杂志, 2020,26(6):103-112.

Yi-ting GUO, Yao CHENG, Yun-yun SHAO, et al. Analysis on Metabolites of Major Components of Shaoyao Gancaotang in Urine and Feces of Normal and Polycystic Ovary Syndrome Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 103-112.
郭一婷, 程瑶, 邵云云, 等. 芍药甘草汤主要成分在正常及多囊卵巢综合征大鼠尿液和粪便中的代谢产物分析[J]. 中国实验方剂学杂志, 2020,26(6):103-112. DOI： 10.13422/j.cnki.syfjx.20192156.

Yi-ting GUO, Yao CHENG, Yun-yun SHAO, et al. Analysis on Metabolites of Major Components of Shaoyao Gancaotang in Urine and Feces of Normal and Polycystic Ovary Syndrome Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 103-112. DOI： 10.13422/j.cnki.syfjx.20192156.

摘要

目的：

分析及鉴定芍药甘草汤主要成分在正常及多囊卵巢综合征(PCOS)大鼠尿液和粪便中的代谢产物，探究PCOS对芍药甘草汤代谢的影响。

方法：

雌性SD大鼠随机分为正常组和PCOS组，利用来曲唑溶液灌胃21 d制备PCOS大鼠模型，观察动情周期；采用UHPLC-QTRAP-MS/MS技术并结合LightSight 2.3软件对芍药甘草汤主要成分在正常及PCOS大鼠尿液和粪便中的代谢产物进行分析与鉴定，流动相水-甲醇梯度洗脱，流速0.3 mL·min

-1

，电喷雾离子源，负离子模式。

结果：

药物代谢主要发生Ⅰ相及Ⅱ相反应，正常组大鼠尿液中共检测到代谢物27个，PCOS组大鼠尿液中共检测到代谢物34个；正常组大鼠粪便样品中共检测到代谢物29个，PCOS组大鼠粪便样品中共检测到代谢物27个。

结论：

PCOS大鼠尿液中代谢产物比正常大鼠更具多样性，PCOS疾病状态可能会影响芍药甘草汤有效成分在体内的代谢途径。

Abstract

Objective:

To analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and polycystic ovary syndrome (PCOS) rats

and to explore the effect of PCOS on the metabolism of Shaoyao Gancaotang.

Method:

Female SD rats were randomly divided into normal group and PCOS group. PCOS rat model was prepared by administration of letrozole solution for 21 days

and the estrous cycle of rats was observed. UHPLC-QTRAP-MS/MS technique and LightSight 2.3 software were used to analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and PCOS rats. The mobile phase was water-methanol for gradient elution

the flow rate was 0.3 mL·min

-1

electrospray ion source was employed under negative ion mode.

Result:

Phase Ⅰ and phase Ⅱ reactions mainly occurred in drug metabolism. A total of 27 metabolites were detected in urine of normal rats

and 34 metabolites were detected in urine of PCOS rats. A total of 29 metabolites were detected in feces of normal rats

and 27 metabolites were detected in feces of PCOS rats.

Conclusion:

The metabolites in the urine of PCOS rats are more diverse than those of normal rats

and the disease status of PCOS may affect the

in vivo

metabolic pathway of active ingredients in Shaoyao Gancaotang.

关键词

Keywords

references

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