基于UPLC-Q-TOF-MS/MS技术的红花寄生及其寄主夹竹桃强心苷成分相关性分析

柴子舒; 刘人源; 李立章; 黄蜚颖; 苏本伟; 朱开昕; 李永华

doi:10.13422/j.cnki.syfjx.20192211

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基于UPLC-Q-TOF-MS/MS技术的红花寄生及其寄主夹竹桃强心苷成分相关性分析

资源与质量评价 | 更新时间：2021-02-09

- 基于UPLC-Q-TOF-MS/MS技术的红花寄生及其寄主夹竹桃强心苷成分相关性分析
- Chemical Constitutes of Cardiac Glycosides in Scurrula parasitica and Its Nerium indicum by UPLC-Q-TOF-MS/MS
- 中国实验方剂学杂志 2019年25卷第22期页码：138-146
- 作者机构：
  
  1.广西中医药大学，南宁　 530200；
  2.钦州市中医药研究所，广西　钦州　 535000
- 作者简介：
  
  柴子舒，在读硕士，从事中药质量控制与中药资源开发研究，Tel：0771-4928552，E-mail：919552139@qq.com
  李永华，博士，研究员，从事中药质量控制与中药资源开发研究，Tel：0771-4928552，E-mail：liyonghua185@126.com
- 基金信息：
  
  国家自然科学基金项目(81660669;81173537);广西自然科学基金项目(2017GXNSFAA198316);广西一流学科建设项目重点课题(2018XK027);广西中医药大学中药学优势学科建设专项(ZYXZ2015002);广西中医药大学中药提取与质量分析重点实验室开放课题(J1700206);广西卫生厅中医药科技专项(GZZY13-39);广西自治区研究生科研创新项目(YCSW2017172;YCSW2018170)
- DOI：10.13422/j.cnki.syfjx.20192211
  中图分类号： R284.2; R289; R22; R2-031
- 收稿日期：2019-03-27，
  
  网络出版日期：2019-08-08，
  
  纸质出版日期：2019-11-20
- 稿件说明：
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柴子舒, 刘人源, 李立章, 等. 基于UPLC-Q-TOF-MS/MS技术的红花寄生及其寄主夹竹桃强心苷成分相关性分析[J]. 中国实验方剂学杂志, 2019,25(22):138-146.

Zi-shu CHAI, Ren-yuan LIU, Li-zhang LI, et al. Chemical Constitutes of Cardiac Glycosides in Scurrula parasitica and Its Nerium indicum by UPLC-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(22): 138-146.
柴子舒, 刘人源, 李立章, 等. 基于UPLC-Q-TOF-MS/MS技术的红花寄生及其寄主夹竹桃强心苷成分相关性分析[J]. 中国实验方剂学杂志, 2019,25(22):138-146. DOI： 10.13422/j.cnki.syfjx.20192211.

Zi-shu CHAI, Ren-yuan LIU, Li-zhang LI, et al. Chemical Constitutes of Cardiac Glycosides in Scurrula parasitica and Its Nerium indicum by UPLC-Q-TOF-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(22): 138-146. DOI： 10.13422/j.cnki.syfjx.20192211.

摘要

目的：

建立超高效液相色谱-飞行时间质谱联用技术(UPLC-Q-TOF-MS/MS)的红花寄生及其寄主夹竹桃强心苷成分鉴定方法，以桂花树寄主的红花寄生及其寄主桂花树样品为对照，通过与强心苷对照品或文献报道比对，鉴定其中的强心苷化学成分，分析红花寄生及其寄主夹竹桃强心苷成分的相关性，并且评价寄主对桑寄生药材质量影响。

方法：

采集红花寄生及其寄主夹竹桃样品，用桂花寄主及其红花寄生为对照样品，样品用70%乙醇超声提取。采用ACQUITY UPLC HSS T3 C

色谱柱(2.1 mm×100 mm，1.8 μm)，以流动相0.1%甲酸水-乙腈梯度洗脱，流速0.6 mL·min

－1

，柱温40 ℃，进样量0.5 μL。以 Masslynx4.1软件分析数据，根据正、负离子模式质谱数据及元素组成分析，结合强心苷对照品和相关文献，对红花寄生及其寄主夹竹桃强心苷成分进行鉴定，并分析各成分之间的相关性。

结果：

共鉴定出26个强心苷成分，其中夹竹桃寄主鉴定出强心苷成分25个，夹竹桃寄主的红花寄生鉴定出强心苷成分5个，桂花寄主及其红花寄生没有强心苷。

结论：

UPLC-Q-TOF-MS/MS技术能快速、准确、全面地鉴定夹竹桃及其红花寄生强心苷成分，红花寄生本身不含强心苷，其强心苷为来自其寄主夹竹桃。

Abstract

Objective:

To establish a method for identifying cardiac glycosides in

Scurrula parasitica

and its

Nerium indicum

host by UPLC-Q-TOF-MS/MS. With safflower parasitoids with sweet-scented osmanthus trees as the host and their host osmanthus tree samples used for control

the chemical constituents of the cardiac glycosides were identified by comparison between the cardiac glycoside reference substances and literatures

so as to analyze the correlation between the safflower parasitoid and its host oleander glycoside components

and evaluate the host's impact on the quality of Taxilli Herba.

Method:

Samples of

parasitica

(parasitic on

indicum

and

Osmanthus fragrans

)

indicum

and

fragrans

were collected. Samples of

parasitica

and its

fragrans

host were taken for control. All of the samples were extracted through ultrasonic extraction with 70%ethanol. ACQUITY UPLC HSS T3 C

(2.1 mm×100 mm

1.8 μm) column was adopted with mobile phase A comprising 0.1%formic acid water and mobile phase B comprising acetonitrile for gradient elution. The sample size was 0.5 μL. The flow rate was 0.6 mL·min

-1

. The column temperature was maintained at 40 ℃. MassLynx V4.1 software was used to analyze the data. Identification and correlation of chemical constitute of cardiac glycosides in

parasitica

and its

indicum

host were performed through analysis on cardiac glycosides reference substances

relevant literatures

elemental composition of compounds and positive and negative ion mode mass spectrometry data.

Result:

A total of 26 compounds of cardiac glycoside were identified

including 25 compounds of cardiac glycoside from

indicum

host

and 5 compounds of cardiac glycoside from

parasitic

(parasitic on

indicum

). none of cardiac glycosides were found in

parasitica

(parasitic on

fragrans

) and its

fragrans

host.

Conclusion:

It was rapid

accurate and comprehensive to identify cardiac glycosides in

parasitica

(parasitic on

indicum

) and its

indicum

host by UPLC-Q-TOF-MS/MS.

parasitica

itself does not contain cardiac glycosides

its host may impact the quality of

parasitica

by delivering cardiac glycosides

a kind of its characteristic compound.

关键词

Keywords

references

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