Objective:

2

To study the chemical constituents from the aerial part of

Allium sativum

.

Method:

2

The aerial part of

A

.

sativum

was treated by heating reflux extraction with 70%ethanol. The combined extracting solution was treated with decompressed recovery solvent to obtain the total extract. The total extract's thick paste was suspended with an appropriate amount of distilled water

and then extracted with petroleum ether

ethyl acetate and water-saturated

n

-butyl alcohol to obtain petroleum ether extraction fraction

ethyl acetate extraction fraction and

n

-butyl alcohol extraction fraction. The

n

-butyl alcohol extraction fraction was isolated and purified by positive phase silica gel column chromatography

ODS column chromatography and preparative HPLC

etc. The structures of the chemical constituents were elucidated by means of physicochemical properties and spectroscopic analysis

and spectral data

like

1

H-NMR

13

C-NMR

DEPT

HMBC and HR-ESI-MS.

Result:

2

Seven compounds were isolated and identified as 2-methoxy-phenol-1-

O

-

α

-

L

-arabinopyranosyl-(1→6)-

β

-

D

-glucopyranoside(1)

phenyl-ethanol-4-

O

-

β

-

D

-xylopyranosyl-(1→6)-

β

-

D

-glucopyranoside(2)

trans

-resveratrol 3-

O

-

β

-

D

-glucopyranoside(3)

stigmasterol(4)

β

-sitosterol(5)

stigmasterol-3-

O

-

β

-

D

-glucopyranoside(6) and daucosterol(7).

Conclusion:

2

Compound 1is a new phenolic glycoside named dasuanxinoside J

and compounds 2-7 are isolated from

A

.

sativum

for the first time.