Objective:

2

To investigate gastrointestinal regulation of red yeast rice on spleen deficient dyspepsia mice

and provide reference for clarifying mechanism of invigorating spleen to promote digestion of fermented traditional Chinese medicine.

Method:

2

Mice were randomly divided into 5 groups

including the blank group

the model group

the unfermented red yeast rice treatment group (1.17 g·kg

-1

·d

-1

)

the fermented red yeast rice treatment group (1.17 g·kg

-1

·d

-1

)

the domperidone group (3.9 mg·kg

-1

·d

-1

)

ten mice in each group were continuous intragastric administration for 10 days. The effects of red yeast rice before and after fermentation on the general behavior

body weight

food intake and gastrointestinal function of spleen deficient dyspepsia mice were compared. The effects of red yeast rice on the gastrointestinal hormone [motilin (MTL)

gastrin (GAS)

5-hydroxytryptamine (5-HT)

vasoactive intestinal peptide (VIP)]

levels in serum of spleen deficient dyspepsia mice were determined by enzyme-linked immunosorption assay (ELISA). The regulation function of red yeast rice on the pathological changes of gastrointestinal tissue of spleen deficient dyspepsia mice was investigated by hematoxylin-eosin (HE) staining. The effect of red yeast rice on gut microbiota of spleen deficient dyspepsia mice was investigated

the primers were designed on bacterial 16S rRNA V3-V4 region sequences and Illumina Miseq platform was used for high-throughput sequencing.

Result:

2

Compared with the model group

fermented red yeast rice could recover the body weight and food intake

reduce gastric residual rate (

P

<

0.01) and enhance the small intestinal propulsive rate (

P

<

0.01) of spleen deficient dyspepsia mice. Red yeast rice could increase the gastrointestinal hormone levels of MTL and 5-HT

while reduce the VIP level of spleen deficient dyspepsia mice. Red yeast rice could restore the light congestion and local structural disorder glands of gastric mucosa of spleen deficient dyspepsia mice. Red yeast rice could restore the pathological changes of intestinal tissue of spleen deficient dyspepsia mice by means of increasing the length of intestinal villus and the thickness of mucosa. Red yeast rice could restore the structure of the gut microbiota of spleen deficient dyspepsia mice by means of reducing the relative abundance of pathogenic microorganisms such as

Escherichia

-

Shigella

while increasing the relative abundance of the beneficial bacteria such as

Lactobacillus

and

Bifidobacterium

.

Conclusion:

2

Fermented red yeast rice can enhance the gastrointestinal function of spleen deficient dyspepsia mice

the mechanism may be related to regulating gastrointestinal hormone level

improving histopathology of gastrointestinal tissue and restoring intestinal flora structure.