欧洲花楸病程相关蛋白基因<italic style="font-style: italic">SaPR</italic>1-<italic style="font-style: italic">like</italic>的克隆及序列分析

刘亚辉; 李佳兴; 王升; 王铁霖; 袁杰; 郭兰萍

doi:10.13422/j.cnki.syfjx.20192348

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欧洲花楸病程相关蛋白基因SaPR1-like的克隆及序列分析

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- 欧洲花楸病程相关蛋白基因SaPR1-like的克隆及序列分析
- Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia
- 中国实验方剂学杂志 2019年25卷第21期页码：118-123
- 作者机构：
  
  1.中国中医科学院　中药资源中心　道地药材国家重点实验室培育基地，北京　 100700；
  2.中国中医科学院　博士后科研流动站，北京　 100700
- 作者简介：
  
  刘亚辉，博士，助理研究员，从事中药资源研究，E-mail：liuyahui2049@163.com
  郭兰萍，博士，研究员，从事中药资源研究，Tel：010-64011944，E-mail：glp01@126.com
- 基金信息：
  
  国家自然科学基金重大项目(81891014);国家自然科学基金项目(81703655);国家重点基础研究发展计划项目(2017YFC1700701);中国博士后科学基金项目(2017M621040)
- DOI：10.13422/j.cnki.syfjx.20192348
  中图分类号： R22; R931; R282; Q523; Q2; Q342
- 收稿日期：2019-07-17，
  
  网络出版日期：2019-08-08，
  
  纸质出版日期：2019-11-05
- 稿件说明：
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刘亚辉, 李佳兴, 王升, 等. 欧洲花楸病程相关蛋白基因SaPR1-like的克隆及序列分析[J]. 中国实验方剂学杂志, 2019,25(21):118-123.

Ya-hui LIU, Jia-xing LI, Sheng WANG, et al. Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(21): 118-123.
刘亚辉, 李佳兴, 王升, 等. 欧洲花楸病程相关蛋白基因SaPR1-like的克隆及序列分析[J]. 中国实验方剂学杂志, 2019,25(21):118-123. DOI： 10.13422/j.cnki.syfjx.20192348.

Ya-hui LIU, Jia-xing LI, Sheng WANG, et al. Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(21): 118-123. DOI： 10.13422/j.cnki.syfjx.20192348.

摘要

目的：

探究欧洲花楸类病程相关蛋白1(SaPR1-like)在响应生物胁迫中的功能。

方法：

克隆得到了

SaPR

基因全长序列，并对该序列进行生物信息学分析；通过实时荧光定量聚合酶链式反应(PCR)方法检测

SaPR

在欧洲花楸悬浮细胞响应harpin蛋白胁迫后的表达特征。

结果：

克隆得到的

SaPR

基因包含1个完整开放阅读框，编码161个氨基酸。该编码蛋白质亲水性强且稳定，具有跨膜结构和信号肽，属于分泌蛋白。氨基酸序列比对分析发现，SaPR1-like蛋白C端具有高度保守结构域[富含半胱氨酸的分泌蛋白、抗原5和致病性相关蛋白1肽段(CAPE)]，推测其在欧洲花楸响应生物胁迫中发挥作用。harpin蛋白诱导欧洲花楸悬浮细胞24 h后，

SaPR

基因表达量显著提高。

结论：

克隆得到了欧洲花楸

SaPR

基因，并证明其在响应生物胁迫时表达和积累，可为进一步阐释欧洲花楸响应生物胁迫机制奠定基础和提供参考。

Abstract

Objective:

To explore the functions of pathogenesis related protein 1-like (PR1-like) in suspension cell of

Sorbus aucuparia

(SaPR1-like) under biotic stress.

Method:

The full sequence of

SaPR

gene was cloned and analyzed by multiple bioinformatic tools. The expression of

SaPR

in the suspension cell of

aucuparia

in response to harpin protein stress was analyzed by real-time fluorescence quantitative polymerase chain reaction (PCR).

Result:

SaPR

gene was identified and cloned

which contained a complete open reading frame and encoded 161 amino acids. The coding protein was hydrophilic and stable

had transmembrane structure and signal peptide

and belonged to secretory protein. Amino acid sequence alignment data indicated that the C-terminal of SaPR1-like contained a highly conserved domain [cysteine-rich secretory protein

antigen 5

and pathogenesis-related 1 peptides (CAPE)]

which could induce defense genes to produce immune responses against biotic stresses.

SaPR

gene expression was significantly increased after harpin protein induced suspension cell of

aucuparia

for 24 h.

Conclusion:

SaPR

gene derived from the

1 family is found to induce significant responses to biotic elicitors in

aucuparia

. This study highlights a role for PR1 in immune signaling and suggests the potential application of PR1 in efforts to defeat biotic stress in plants.

关键词

Keywords

references

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欧洲花楸病程相关蛋白基因SaPR1-like的克隆及序列分析

Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia

DOI：10.13422/j.cnki.syfjx.20192348

摘要

Abstract

关键词

Keywords

references