基于网络药理学探讨断藤益母汤治疗类风湿关节炎的作用机制

王强; 刘敏莹; 韩隆胤; 郭文杰; 刘小宝; 潘东梅; 李楠; 林昌松

doi:10.13422/j.cnki.syfjx.20192404

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基于网络药理学探讨断藤益母汤治疗类风湿关节炎的作用机制

数据挖掘 | 更新时间：2021-02-09

- 基于网络药理学探讨断藤益母汤治疗类风湿关节炎的作用机制
- Mechanism of Duanteng Yimutang in Treating Rheumatoid Arthritis Based on Network Pharmacology Approach
- 中国实验方剂学杂志 2019年25卷第24期页码：150-158
- 作者机构：
  
  1.广州中医药大学　第一临床医学院，广州　 510405；
  2.广州中医药大学　第一附属医院，广州　 510405；
  3.南方医科大学　中医药学院，广州　 510405；
  4.暨南大学　中医学院，广州　 510405
- 作者简介：
  
  王强，在读博士，从事中医药防治风湿病研究，E-mail：wangqiang5250@163.com
  林昌松，博士生导师，从事中医药防治风湿病研究，Tel：020-36598915，E-mail：linchs999@163.com
- 基金信息：
  
  国家自然科学基金项目(81573930;81774262);广东省自然科学基金项目(2017A030311009;2018A0303130112);中央高校基本科研业务费专项(21618335)
- DOI：10.13422/j.cnki.syfjx.20192404
  中图分类号： R2-0; R22; R285.5; R289
- 收稿日期：2019-04-14，
  
  网络出版日期：2019-09-05，
  
  纸质出版日期：2019-12-20
- 稿件说明：
移动端阅览
王强, 刘敏莹, 韩隆胤, 等. 基于网络药理学探讨断藤益母汤治疗类风湿关节炎的作用机制[J]. 中国实验方剂学杂志, 2019,25(24):150-158.

Qiang WANG, Min-ying LIU, Long-yin HAN, et al. Mechanism of Duanteng Yimutang in Treating Rheumatoid Arthritis Based on Network Pharmacology Approach[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(24): 150-158.
王强, 刘敏莹, 韩隆胤, 等. 基于网络药理学探讨断藤益母汤治疗类风湿关节炎的作用机制[J]. 中国实验方剂学杂志, 2019,25(24):150-158. DOI： 10.13422/j.cnki.syfjx.20192404.

Qiang WANG, Min-ying LIU, Long-yin HAN, et al. Mechanism of Duanteng Yimutang in Treating Rheumatoid Arthritis Based on Network Pharmacology Approach[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(24): 150-158. DOI： 10.13422/j.cnki.syfjx.20192404.

摘要

目的：

断藤益母汤在类风湿关节炎中疗效显著，但其成分复杂，作用机制不明。使用网络药理学方法对断藤益母汤的分子机制进行研究，寻找断藤益母汤的有效成分、治疗靶标和信号通路具有一定价值。

方法：

根据药代动力学参数在中药系统药理学数据库及分析平台(TCMSP)和中医综合数据库(TCMID)筛选药物成分。在Drugbank数据库和TTD数据库挖掘药物和疾病靶标，使用可视化网络药理学Cytoscap工具构建药物-靶点-通路网络，探讨断藤益母汤中成分的作用机制。

结果：

发现断藤益母汤的11个有效成分可能靶向42个类风湿关节炎相关靶点，如白细胞介素-1(IL-1)，白细胞介素-6(IL-6)，肿瘤坏死因子(TNF)，环氧化酶2(COX2)，基质金属蛋白酶-1(MMP-1)，基质金属蛋白酶-3(MMP-3)，诱导型一氧化氮合酶(NOS

)等。参与了包括肿瘤坏死因子(TNF)信号通路，白细胞介素-17(IL-17)通路，辅助性T细胞17(Th17)分化通路，风湿关节炎通路、核转录因子-

B (NF-

B)通路、破骨细胞分化通路、卵巢类固醇生成通路等多条通路。

结论：

断藤益母汤可能主要通过多条炎症相关信号通路对炎性细胞因子进行调控，从而对类风湿关节炎起到抗炎和免疫调控作用。

Abstract

Objective:

Duanteng Yimutang(DTYMT) has a significant effect in treating rheumatoid arthritis

but its composition is complex and its mechanism is not clear. It is worthwhile to use network pharmacology approach to find active components

therapeutic targets and signal pathways of DTYMT.

Method:

The drug composition was selected according to the pharmacokinetic parameters in the pharmacology database

the analysis platform (TCMSP) and the TCM integrated database (TCMID) of the Traditional Chinese Medicine System. The drug and disease targets were excavated in the Drugbank database and the Therapeutic Target Database (TTD)

and the drug-target-pathway network was constructed by network pharmacology tool Cytoscap

in order to explore the mechanism of the action of the components in the DTYMT.

Result:

It was found that 11 effective components of DTYMT could target 42 proteins in rheumatoid arthritis

such as interleukin-1 (IL-1)

interleukin-6 (IL-6)

tumor necrosis factor (TNF)

cyclooxygenase-2 (COX2)

matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-3 (MMP-3)

and inducible nitric oxide synthase (NOS2). Various pathways

including tumor necrosis factor (TNF) signaling pathway

interleukin-17 (IL-17) pathway

helper T cell 17 (Th17) differentiation pathway

rheumatic arthritis pathway

nuclear factor

B (NF-

B) pathway

osteoclast differentiation pathway

and ovarian steroid production pathway

were involved.

Conclusion:

DTYMT may be used to regulate inflammatory cytokines mainly through multiple inflammatory-related signal pathways

so as to play anti-inflammatory and immunoregulatory roles in the treatment of rheumatoid arthritis.

关键词

Keywords

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