Objective:

2

To determine the relationship between the characters and the main components of Chelidonii Herba pieces.

Method:

2

The main components of the samples were determined by HPLC

and the characters of Chelidonii Herba Pieces were evaluated by sensory evaluation. The correlation between the characters and components was calculated by correlation formula.

Result:

2

The content of 6 components

such as chelidonine

was determined by HPLC. Based on the result of sensory evaluation

there was a certain correlation between the characters and the components. Character descriptions that " some have visible white powder

some have white pubescence

and sometimes they have visible yellow florets" had a very low similarity with total alkali

indicating that these characters and total alkali content was not related. The similarity between the description of " hollow stem" had a high similarity with total alkali content

indicating that the amount of stem was related to the total alkali content. The character description of " more broken leaves" was negatively correlated with total alkaloids in the similarity

which indicated that the content of total alkaloids was less when there were more leaves(or more broken leaves)

otherwise

the content of total alkaloids was relatively higher.

Conclusion:

2

The established HPLC method is simple and feasible. This study objectively quantifies the descriptions of Chelidonii Herba pieces characters

correlates them with the main components of Chelidonii Herba pieces

and then preliminarily judges the quality of Chelidonii Herba pieces according to the appearance of the characters

which provides a theoretical basis for the identification of Chelidonii Herba pieces in the market by experience

and ideas for the study of the characters of other traditional Chinese medicines.