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1.黑龙江中医药大学 药学院,哈尔滨 150040
2.东北林业大学 林学院,哈尔滨 150040
3.广东药科大学 附属第一医院,广州 510080
[第一作者] 金司阳,在读博士,从事中药资源研究,E-mail:jsy_0451@126.com
*王振月,教授,从事中药资源开发与生物技术研究,E-mail: wangzhen_yue@163.com
收稿日期:2019-03-02,
网络出版日期:2019-09-18,
纸质出版日期:2020-02-20
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金司阳, 刘寒, 杨立学, 等. 植物-土壤反馈对刺五加幼苗抗氧化酶系统的影响[J]. 中国实验方剂学杂志, 2020,26(4):162-166.
Si-yang JIN, Han LIU, Li-xue YANG, et al. Effect of Plant and Soil Feedback on Antioxidant System of Acanthopanax senticosus[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 162-166.
金司阳, 刘寒, 杨立学, 等. 植物-土壤反馈对刺五加幼苗抗氧化酶系统的影响[J]. 中国实验方剂学杂志, 2020,26(4):162-166. DOI: 10.13422/j.cnki.syfjx.20200112.
Si-yang JIN, Han LIU, Li-xue YANG, et al. Effect of Plant and Soil Feedback on Antioxidant System of Acanthopanax senticosus[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 162-166. DOI: 10.13422/j.cnki.syfjx.20200112.
目的:
2
该文研究了植物土壤反馈对刺五加幼苗抗氧化酶系统的影响,有利于阐述植物-土壤反馈对于刺五加幼苗生长中抗氧化酶系统的变化,为揭示植物-土壤反馈的原因提供理论依据。
方法:
2
通过温室盆栽试验,分别对未种植过刺五加的土壤(1组),连续3年种植刺五加的土壤(2组)和多年种植刺五加的土壤(3组)进行株高,叶色值(SPAD),抗氧化酶系统相关指标[蛋白质,超氧化物歧化酶(SOD),过氧化氢酶(CAT),过氧化物酶(POD),抗坏血酸过氧化物酶(APX),丙二醛(MDA)]和产量、生长相关指标进行测定。
结果:
2
未种植过刺五加的土壤(1组),连续三年种植刺五加的土壤(2组)和多年种植刺五加的土壤(3组)在后续种植的刺五加幼苗的株高,叶色值(SPAD),生物量,蛋白,SOD,CAT,POD,APX,MDA有显著性差异,ACP活性无显著性差异。其中未种植过刺五加的土壤(1组)的刺五加幼苗MDA,CAT,POD和SOD要高于种植过刺五加的土壤(2,3组)的刺五加幼苗,而蛋白和APX要低于种植过刺五加的土壤(2,3组)的刺五加幼苗。
结论:
2
植物和土壤在刺五加幼苗生长过程中呈现负反馈调节,降低幼苗抗氧化酶活性。因此,未种植过刺五加的土壤对刺五加幼苗的生长更有优势。研究结果为阐述植物-土壤反馈对刺五加生长的影响提供研究基础,并为农田栽培刺五加技术标准提供理论依据和技术支持。
Objective:
2
To study the effect of plant-soil feedback on the antioxidant enzyme system of
Acanthopanax senticosus
seedlings
in order to elucidate the changes of plant-soil feedback on the antioxidant enzyme system of
A
.
senticosus
seedlings
and provide theoretical basis for revealing the reasons of plant-soil feedback.
Method:
2
Through the greenhouse pot experiment
plant height
leaf color value (SPAD)
antioxidant enzymes [protein
superoxide dismutase(SOD)
catalase(CAT)
peroxidase(POD)
aseorbateperoxidase(APX)
malondialdehyde(MDA)] and yield and growth related inde
xes of soil without
A
.
senticosus
(group 1)
soil with A. senticosus (group 2) for three consecutive years and soil with
A
.
senticosus
(group 3) for many years were measured respectively.
Result:
2
There were significant differences in plant height
SPAD
protein
SOD
CAT
POD
APX and MDA between seedlings of
A
.
senticosus
planted for three consecutive years (group 1)
two successive years (group 2) and three successive years (group 3). The biomass
MDA
CAT
POD and SOD of
A
.
senticosus
seedlings in the soil without
A
.
senticosus
(group 1) were higher than those in the soil with
A
.
senticosus
(group 2 and 3)
while the protein and APX were lower than those in the soil with
A
.
senticosus
(group 2 and 3).
Conclusion:
2
Plant and soil shows negative feedback regulation during the growth of
A
.
senticosus
seedlings
which reduced the activity of antioxidant enzymes. Therefore
the soil without planting
A
.
senticosus
has more advantages for the growth of
A
.
senticosus
seedlings. The results provide a basis for explaining the effect of plant-soil feedback on the growth of
A
.
senticosus
and a theoretical basis and technical support for the technical standards of
A
.
senticosus
cultivation in farmland.
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