基于网络药理学探讨黄连-吴茱萸治疗胃癌的作用机制

郑琦; 侯炜

doi:10.13422/j.cnki.syfjx.20200122

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基于网络药理学探讨黄连-吴茱萸治疗胃癌的作用机制

数据挖掘 | 更新时间：2021-02-09

- 基于网络药理学探讨黄连-吴茱萸治疗胃癌的作用机制
- Molecular Mechanism of Coptidis Rhizoma-Euodiae Fructus for Gastric Cancer Based on Network Pharmacology
- 中国实验方剂学杂志 2020年26卷第7期页码：204-210
- 作者机构：
  
  中国中医科学院　广安门医院，北京　 100053
- 作者简介：
  
  [第一作者]　郑琦，博士，助理研究员，从事中医药防治肿瘤的基础研究，E-mail：fiona19880607@sina.cn
  *侯炜，硕士，主任医师，博士生导师，从事中医药防治肿瘤方面的临床和基础研究，E-mail：houwei1964@163.com
- 基金信息：
  
  国家自然科学基金项目(81673754)
- DOI：10.13422/j.cnki.syfjx.20200122
  中图分类号： R22; R242; R2-031;R285.5
- 收稿日期：2019-05-05，
  
  网络出版日期：2019-09-12，
  
  纸质出版日期：2020-04-05
- 稿件说明：
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郑琦, 侯炜. 基于网络药理学探讨黄连-吴茱萸治疗胃癌的作用机制[J]. 中国实验方剂学杂志, 2020,26(7):204-210.

Qi ZHENG, Wei HOU. Molecular Mechanism of Coptidis Rhizoma-Euodiae Fructus for Gastric Cancer Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 204-210.
郑琦, 侯炜. 基于网络药理学探讨黄连-吴茱萸治疗胃癌的作用机制[J]. 中国实验方剂学杂志, 2020,26(7):204-210. DOI： 10.13422/j.cnki.syfjx.20200122.

Qi ZHENG, Wei HOU. Molecular Mechanism of Coptidis Rhizoma-Euodiae Fructus for Gastric Cancer Based on Network Pharmacology[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 204-210. DOI： 10.13422/j.cnki.syfjx.20200122.

摘要

目的：

筛选黄连-吴茱萸配伍对胃癌治疗的主要活性成分，预测两者共同活性成分的作用靶点，建立药物活性成分-靶基因网络，进一步探讨药对黄连-吴茱萸对胃癌治疗的潜在作用机制。

方法：

采用中药系统药理学技术平台(TCMSP)，筛选黄连-吴茱萸的潜在活性化学物质，借助基因组注释(Genecards)数据库平台，预测和筛选胃癌的作用靶点，采用Cytoscape(3.7.1)软件，构建“药物-活性化学物质-疾病-作用靶点”网络图，借助String数据库平台构建蛋白质相互作用网络。最后利用Bioconductor平台和R语言进行基因本体(GO)富集分析和基因相互作用(KEGG)通路分析。

结果：

通过筛选，得到黄连的潜在活性成分14个，吴茱萸的潜在活性成分15个，共涉及与胃癌相关作用靶点127个。黄连-吴茱萸-胃癌共同靶点33个，主要通过调控PTGS2，PTGS1，AR，RXRA，NOS2等靶基因及细胞凋亡，p53信号通路，白细胞介素(IL)-17信号通路等发挥对胃癌的治疗作用。

结论：

黄连-吴茱萸对胃癌的治疗作用体现了中药多成分-多靶点的特点，阐明了药对黄连-吴茱萸对胃癌治疗的作用机制与物质基础的科学内涵。

Abstract

Objective:

To identify the main active components of Coptidis Rhizoma and Euodiae Fructus for the treatment of gastric cancer

predict the targets of the common active components in these two herbs

establish the network of active drug components-target genes

and further explore the potential mechanism and effect of Coptidis Rhizoma-Euodiae Fructus for the treatment of gastric cancer.

Method:

The active components of Coptidis Rhizoma-Euodiae Fructus were screened by Traditional Chinese Medicine Systems Pharmacology Database (TCMSP)

gastric cancer targets were predicted and screened by Genecard database platform

" drug-active ingredient-disease-targets" networks were built by Cytoscape (3.7.1) software

and protein interaction networks were built by String database platform. Finally

Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed by using Bioconductor platform and R language.

Result:

The 14 potential active components of Coptidis Rhizoma and 15 potential active components of Euodiae Fructus were obtained

involving 127 targets related to gastric cancer. There were 33 common targets for Coptidis Rhizoma-Euodiae Fructus-gastric cancer

which played a therapeutic role in gastric cancer mainly by regulating target genes such as PTGS2

PTGS1

RXRA and NOS2

as well as cell apoptosis

p53 signaling pathway and interleukin (IL)-17 signaling pathway.

Conclusion:

The therapeutic mechanism of Coptidis Rhizoma-Euodiae Fructus reflects the multi-component

multi-target and multi-pathway characteristics of traditional Chinese medicines and provides the scientific basis for further study and the material basis of Coptidis Rhizoma-Euodiae Fructus against gastric cancer.

关键词

Keywords

references

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