狼毒大戟抗肿瘤活性成分

马玉坤; 马天成; 孙宇; 刘雷; 郭丽娜; 孙珈; 刘琦; 韩翠翠

doi:10.13422/j.cnki.syfjx.20200216

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狼毒大戟抗肿瘤活性成分

药学基础 | 更新时间：2021-02-09

- 狼毒大戟抗肿瘤活性成分
- Anti-tumor Constituents from Rhizome of Euphorbia fischeriana
- 中国实验方剂学杂志 2020年26卷第7期页码：156-164
- 作者机构：
  
  齐齐哈尔医学院，黑龙江齐齐哈尔　 161006
- 作者简介：
  
  [第一作者]　马玉坤，硕士，实习研究员，从事天然药物化学成分研究，Tel：0452-2663371，E-mail：769650146@qq.com
  *孙宇，博士，助理研究员，从事天然药物化学成分及质量分析研究，Tel: 0452-2663371，E-mail：zsy5811321@126.com
- 基金信息：
  
  黑龙江省教育厅科学技术研究项目(2017-KYYWF-0702)
- DOI：10.13422/j.cnki.syfjx.20200216
  中图分类号： R289; R284.1; R22; R2-031
- 收稿日期：2019-09-25，
  
  网络出版日期：2019-10-11，
  
  纸质出版日期：2020-04-05
- 稿件说明：
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马玉坤, 马天成, 孙宇, 等. 狼毒大戟抗肿瘤活性成分[J]. 中国实验方剂学杂志, 2020,26(7):156-164.

Yu-kun MA, Tian-cheng MA, Yu SUN, et al. Anti-tumor Constituents from Rhizome of Euphorbia fischeriana[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 156-164.
马玉坤, 马天成, 孙宇, 等. 狼毒大戟抗肿瘤活性成分[J]. 中国实验方剂学杂志, 2020,26(7):156-164. DOI： 10.13422/j.cnki.syfjx.20200216.

Yu-kun MA, Tian-cheng MA, Yu SUN, et al. Anti-tumor Constituents from Rhizome of Euphorbia fischeriana[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 156-164. DOI： 10.13422/j.cnki.syfjx.20200216.

摘要

目的：

筛选狼毒大戟抗肿瘤活性馏分，分离活性单体化合物并对构效关系进行探讨。利用超高效液相色谱-质谱联用技术(UPLC-Q-TOF-MS)对活性馏分的化学成分进行鉴定。

方法：

采用噻唑蓝(MTT)法对狼毒大戟乙醇提取物的石油醚、乙酸乙酯、正丁醇和水萃取部位的抗肿瘤活性进行测试。综合运用多种现代色谱分离手段对活性萃取层石油醚层进行分离。利用核磁(NMR)和质谱(MS)技术鉴定化合物的化学结构。质谱使用ESI离子源，正离子模式下采集数据，结合狼毒大戟相关文献以及对照品信息进行数据分析，解析活性馏分的化合物结构。

结果：

从活性馏分层石油醚层分离得到6个单体化合物，分别为岩大戟内酯A，岩大戟内酯B，17-羟-岩大戟内酯A，17-羟-岩大戟内酯B，euphopilolide，atis-16-en-13(s)-hydroxy-3，14-dione。对这些化合物的抗肿瘤活性的构效关系进行了探讨。利用UPLC-Q-TOF-MS对狼毒大戟乙醇提取物的石油醚萃取部位化学成分进行鉴定，共鉴定化合物

个，其中二萜类成分19个，酚类成分2个，脂肪酸类成分1个，三萜类成分1个。峰18和峰21初步鉴定为新化合物。

结论：

狼毒大戟的活性馏分层为石油醚层，其主要成分为二萜类，具有较好的抗肿瘤活性。UPLC-Q-TOF-MS技术能够快速准确地鉴定狼毒大戟活性馏分的化学成分结构，为其质量评价和活性物质基础研究提供了参考。

Abstract

Objective:

To screen out active fraction from

Euphorbia fischeriana

separate active components from

fischeriana

and explore structure-activity relationships

in order to analyze and identify chemical compositions of petroleum ether fraction from

fischerian

ethanol extract by ultra-performance liquid chromatography with quadrupole-time-of flight mass spectrometry (UPLC-Q-TOF-MS).

Method:

The anti-tumor activities of petroleum ether

ethyl acetate

-butanol and water extracts from

fischeriana

were tested by methyl thiazolyl tetrazolium(MTT)method. A variety of modern chromatographic separation methods were used to separate active compounds from petroleum ether layer. Compounds were isolated. Their structures were identified by NMR technique. The structure-activity relationships between anti-tumor activities and structures of compounds were investigated. UPLC-Q-TOF-MS technique was used to identify the structures of petroleum ether extract from

fischeriana

. Mass spectrometry was performed in the positive ion mode using ESI ion sources.

Result:

Six compounds were isolated from petroleum ether fraction. They were jolkinolide A

jolkinolide B

17-hydroxyjolkinolide A

17-hydroxyjolkinolide B

euphopilolide and atis-16-en-13(s)-hydroxy-3

14-dione. A total of 23 peaks were identified based on the comparison of retention times

accurate masses and fragmentation patterns with available standard compounds and literatures. Among them

there were 19 diterpenoids

2 polyphenols

1 fatty acid and 1 triterpenoid. Peaks No.18 and No.21 were tentatively identified as new compounds.

Conclusion:

The petroleum ether fraction showed a potential anti-tumor activity. The structure-activity relationships were discussed. UPLC-Q-TOF-MS technology can be used to quickly and accurately identify the structures

so as to provide a reference for its quality evaluation and active ingredient research.

关键词

Keywords

references

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