Objective

2

To observe the clinical efficacy of modified Zhibo Dihuangwan on henoch-schonlein purpura nephritis with deficiency of liver and kidney yin in children (HSPN) and its effect on immune inflammatory response and hypercoagulable state.

Method

2

Totally 120 patients were randomly divided into observation group (60 cases) and control group (60 cases) by random number table. Patients in two group was orally given prednisolone acetate tablets

1.5-2 mg·kg

-1

·d

-1

2 times. Four weeks later

the drug was taken orally every other day

and the dosage decreased gradually after 4 weeks. Besides

patients in control group was intravenously dripped with cyclophosphamide

8-12 mg·kg

-1

·d

-1

for 2 days

and stopped for 2 weeks before another treatment course. The treatment lasted for 6 months. In the control group

Dabuyin Wan was taken orally

3 g/time

3 times/d.Patients in observation group was also added with modified Zhibo Dihuang Wan

1 doe/day. The treatment lasted for 6 months. Urine routine was tested once a month

and disappearance time and rate of hematuria and albuminuria were recorded. The 24 h urine protein quantification

levels of microalbuminuria (mAlb) and urinary

β

2

-microglobulin (

β

2

-MG) were assessed before and after treatment. Furthermore

deficiency of liver and kidney Yin was scored

and levels of T lymphocyte subsets (CD3

+

CD4

+

CD8

+

CD4

+

/CD8

+

)

fibrinogen (FIB)

D

-dimer (

D

-D)

fibrin degradation products (FDP)

interleukin-2 (IL-2)

interferon-

γ

(IFN-

γ

)

interleukin-4 (IL-4)

interleukin-10 (IL-10) were detected.

Result

2

The clinical efficacy in observation group was superior to that in control group (

Z

=2.078，

P

<

0.05). Disappearance times of hematuria and albuminuria of children in observation group were shorter than those in control group (

P

<

0.01). The disappearance rate of proteinuria in observation group was 90.48%（38/42）

which was higher than 69.77%（30/43） in control group (

χ

2

=5.694，

P

<

0.05). The 24 h urinary protein quantity

mAlb and levels of

β

2

-MG

FIB

D-D and FDP in observation group were lower than those in control group (

P

<

0.01). The levels of CD3

+

CD4

+

IL-2 and IFN-

γ

and the ratio of CD4

+

/CD8

+

in observation group were higher than those in control group (

P

<

0.05)

while the CD8

+

IL-4 and IL-10 were lower than those in control group (

P

<

0.05). The efficacy in observation group was better than that in control group (

Z

=2.106，

P

<

0.05).

Conclusion

2

In addition to conventional western medicine therapy

modified Zhibo Dihuang Wan have an effect on HSPN with deficiency of liver and kidney Yin in children by promoting the disappearance of albuminuria and hematuria

shortening the course of disease

improving T lymphocyte subpopulation

reducing inflammatory reaction and correcting hypercoagulable state of blood

with better clinical efficacy and syndrome effect of traditional Chinese medicine.