龙血通络胶囊对H<sub>2</sub>O<sub>2</sub>诱导PC12细胞损伤的保护作用

陈孝男; 杨爱琳; 赵亚楠; 田颖颖; 屠鹏飞; 胡仲冬; 李军

doi:10.13422/j.cnki.syfjx.20200402

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龙血通络胶囊对H₂O₂诱导PC12细胞损伤的保护作用

药理 | 更新时间：2021-02-09

- 龙血通络胶囊对H₂O₂诱导PC12细胞损伤的保护作用
- Protective Effect of Longxue Tongluo Capsule on H₂O₂-induced Injury of PC12 Cells
- 中国实验方剂学杂志 2020年26卷第7期页码：50-56
- 作者机构：
  
  1.北京中医药大学　中药学院　中药现代研究中心，北京　 100029
  2.中药品质评价北京市重点研究室，北京　 102488
- 作者简介：
  
  [第一作者]　陈孝男，在读硕士，从事中药药效作用机制研究，E-mail：cxnnjucm@163.com
  *胡仲冬，博士，副研究员，从事中药活性成分药理研究，E-mail: huzhongdong@126.com；
  *李军，博士，研究员，从事中药药效物质、作用机制和质量评价研究，E-mail: drlj666@163.com
- 基金信息：
  
  国家自然科学基金项目(81573572);中央高校基本科研业务费专项(2015-JYB-XYQ-004);北京中医药大学基本科研业务在读研究生项目(2019-JYB-XS-048);中华中医药学会青年人才托举工程项目(CACM-2018-（QNRC2-B05）)
- DOI：10.13422/j.cnki.syfjx.20200402
  中图分类号： R2-0; R22; R285.5
- 收稿日期：2019-07-31，
  
  网络出版日期：2019-11-06，
  
  纸质出版日期：2020-04-05
- 稿件说明：
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陈孝男, 杨爱琳, 赵亚楠, 等. 龙血通络胶囊对H₂O₂诱导PC12细胞损伤的保护作用[J]. 中国实验方剂学杂志, 2020,26(7):50-56.

Xiao-nan CHEN, Ai-lin YANG, Ya-nan ZHAO, et al. Protective Effect of Longxue Tongluo Capsule on H₂O₂-induced Injury of PC12 Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 50-56.
陈孝男, 杨爱琳, 赵亚楠, 等. 龙血通络胶囊对H₂O₂诱导PC12细胞损伤的保护作用[J]. 中国实验方剂学杂志, 2020,26(7):50-56. DOI： 10.13422/j.cnki.syfjx.20200402.

Xiao-nan CHEN, Ai-lin YANG, Ya-nan ZHAO, et al. Protective Effect of Longxue Tongluo Capsule on H₂O₂-induced Injury of PC12 Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 50-56. DOI： 10.13422/j.cnki.syfjx.20200402.

摘要

目的：

本文探讨过氧化氢(H

)诱导PC12细胞损伤后，龙血通络胶囊的神经保护作用及其潜在作用机制。

方法：

以500 μmol·L

-1

构建PC12细胞损伤模型，实验分为空白组，模型组(H

500 μmol·L

-1

)，龙血通络胶囊组(LTC，1，2，4 mg·L

-1

)。通过细胞增殖毒性检测(CCK-8)，胞内活性氧(ROS)水平，线粒体膜电位水平(JC-1)，细胞凋亡率以及蛋白免疫印迹法(Western blot)评价龙血通络胶囊对H

诱导PC12细胞损伤的神经保护作用。

结果：

与空白组比较，模型组的细胞存活率显著降低(

0.01)，ROS水平显著上升(

0.01)，线粒体膜电位水平下降，细胞凋亡率显著升高(

0.01)，剪切的聚腺苷二磷酸核糖聚合酶(cleaved PARP)水平显著上升(

0.01)。与模型组比较，LTC组(1，2，4 mg·L

-1

)能浓度依赖性升高细胞的存活率(

0.01)，降低ROS水平(

0.05)，维持线粒体膜电位水平，降低细胞的凋亡水平(

0.01)，并显著降低PARP蛋白的剪切水平(

0.01)。

结论：

龙血通络胶囊对H

诱导的PC12细胞损伤具有显著的保护作用，其作用机制可能是通过抑制胞内氧化应激，维持线粒体功能，促进DNA修复等途径进而抑制神经细胞凋亡。

Abstract

Objective:

To investigate the protective effect of Longxue Tongluo capsule (LTC) on H

-induced injury of PC12 cells and its potential mechanism.

Method:

in vitro

injury model in PC12 cells was established with 500 μmol·L

-1

.The experiment was divided into control group

injury model group (H

500 μmol·L

-1

)

and Longxuetongluo capsule group (LTC

4 mg·L

-1

). Cell counting kit-8 (CCK-8) assay

intracellular reactive oxygen species (ROS)

mitochondrial membrane potential

apoptosis of PC12 cells

and Western blot were used to evaluate the protective effect of LTC on PC12 cells induced by H

Result:

Compared with the control group

the cell viability was significantly decreased in the injury model group (

0.01)

intracellular ROS level was significantly increased (

0.01)

mitochondrial membrane potential was decreased

while apoptosis of PC12 cells was significantly increased (

0.01)

and the expression of cleaved poly adenosine diphospho ribose polymerase (PARP) was also increased significantly (

0.01). Compared with the injury model group

pretreatment with LTC at the concentrations of 2 and 4 mg·L for 6 h significantly increased cell viability in PC12 cells exposed to H

(

0.01). Moreover

pretreatment with LTC reduced intracellular ROS level (

0.05)

maintained mitochondrial membrane potential

and inhibited apoptosis of PC12 cells induced by H

in a dose-dependent manner (

0.01). The results of western blotting showed that pretreatment with LTC significantly reduced the expression of cleaved PARP (

0.01).

Conclusion:

LTC exerts a significant protective effect against H

-induced PC12 cells injury through inhibition of neuronal apoptosis by suppressing intracellular oxidative stress

maintaining mitochondrial function

and promoting DNA repair.

关键词

Keywords

references

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Protective Effect of Longxue Tongluo Capsule on H2O2-induced Injury of PC12 Cells

DOI：10.13422/j.cnki.syfjx.20200402

摘要

Abstract

关键词

Keywords

references

龙血通络胶囊对H₂O₂诱导PC12细胞损伤的保护作用

Protective Effect of Longxue Tongluo Capsule on H₂O₂-induced Injury of PC12 Cells