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1.黑龙江中医药大学 药学院,哈尔滨 150040
2.东北林业大学 林学院,哈尔滨 150040
3.广东药科大学 附属第一医院,广州 510080
[第一作者] 金司阳,在读博士,从事中药资源研究,E-mail:jsy_0451@126.com
*王振月,教授,从事中药资源开发与生物技术研究,E-mail:wangzhen_yue@163.com
收稿日期:2019-05-04,
网络出版日期:2019-11-07,
纸质出版日期:2020-02-20
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金司阳, 刘寒, 杨立学, 等. 植物-土壤反馈对刺五加幼苗次生代谢产物的影响[J]. 中国实验方剂学杂志, 2020,26(4):167-173.
Si-yang JIN, Han LIU, Li-xue YANG, et al. Effect of Plant-Soil Feedback on Secondary Metabolites of Acanthopanax senticosus Seedlings[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 167-173.
金司阳, 刘寒, 杨立学, 等. 植物-土壤反馈对刺五加幼苗次生代谢产物的影响[J]. 中国实验方剂学杂志, 2020,26(4):167-173. DOI: 10.13422/j.cnki.syfjx.20200413.
Si-yang JIN, Han LIU, Li-xue YANG, et al. Effect of Plant-Soil Feedback on Secondary Metabolites of Acanthopanax senticosus Seedlings[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 167-173. DOI: 10.13422/j.cnki.syfjx.20200413.
目的:
2
研究植物-土壤反馈对刺五加幼苗根、茎、叶次生代谢产物的影响。
方法:
2
通过温室盆栽试验,分别对未种植过刺五加的土壤(1组),连续3年种植刺五加的土壤(2组)和多年种植刺五加的土壤(3组),分别种植刺五加1年生幼苗,并对其根、茎、叶的次生代谢产物进行分析。
结果:
2
L
-苯丙氨酸,原儿茶酸,刺五加苷B,绿原酸,咖啡酸,刺五加苷E,异嗪皮啶,芦丁,金丝桃苷,槲皮素在多年生长刺五加土壤种植,对刺五加幼苗叶和根均有显著性差异,但在茎中绿原酸和刺五加苷E无显著性差异。其中刺五加苷E,异嗪皮啶,芦丁和金丝桃苷在多年生刺五加土壤种植的幼苗叶中未检出。在刺五加幼苗的根中,多数次生代谢产物呈现正反馈;在刺五加幼苗的茎中,咖啡酸,刺五加苷E,金丝桃苷,槲皮素呈现负反馈;在刺五加幼苗的叶中多数次生代谢产物呈现正反馈。
结论:
2
植物和土壤在刺五加幼苗生长过程不同部位呈现出不同的反馈情况,整体而言,未种植过刺五加的土壤对刺五加幼苗的次生代谢产物更具优势。研究结果为阐述植物-土壤反馈对刺五加的影响提供研究基础,并为人工栽培刺五加提供了理论依据和技术支持。
Objective:
2
To investigate the effects of plant-soil feedback on secondary metabolites in roots
stems and leaves of
Acanthopanax senticosus
seedlings.
Method:
2
One-year-old seedlings of
A
.
senticosus
were planted in the soil where no
A
.
senticosus
had been planted before (group 1)
soil where
A
.
senticosus
had been planted for 3 years (group 2)
and soil where
A
.
senticosus
had been planted for many years in the greenhouse pot experiment
and the secondary metabolites of its roots
stems and leaves were then analyzed.
Result:
2
L
-Phenylalanine
3
4-dihydroxybenzoic acid
syringin
chlorogenic acid
caffeic acid
eleutheroside E
isofraxidin
rutin
hyperoside
and quercetin had significant differences in leaves and roots of
A
.
senticosus
seedlings in the soil of group 3
but there was no significant difference in chlorogenic acid and eleutheroside E in stem. Eleutheroside E
isofraxidin
rutin and hyperoside were not detected in the leaves of seedlings planted in group 3.Most of the secondary metabolites in the roots of
A
.
senticosus
seedlings showed positive feedback
while in the stems of
Acanthopanax senticosus
seedlings
caffeine
A
.
senticosus
glycosides
hypericin and quercetin showed negative feedback
and most of the secondary metabolites in the leaves of
A
.
senticosus
seedlings showed positive feedback.
Conclusion:
2
The plant and soil showed different feedback in different parts of the growth process of
A
.
senticosus
seedlings
and the soil where
A
.
senticosus
had not been planted was more advantageous to the secondary metabolites of
A
.
senticosus
seedlings. The results of the study provide a basis for the study of the effect of plant-soil feedback on the
A
.
senticosus
and provide the theoretical basis and technical support for the artificial cultivation of
A
.
senticosus
.
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