Objective:

2

To investigate the differences and correlations of active ingredients and principal ingredients of steroid saponins in rhizomes of 9 kinds of

Paris

.

Method:

2

The differences and correlations of active ingredients and principal ingredients of steroid saponins in rhizomes of 9 kinds of

Paris

. were qualitatively analyzed by liquid chromatography-mass spectrometry (LC-MS).

Result:

2

PolyphyllinⅠ

polyphyllin Ⅱ

polyphyllin Ⅵ

and polyphyllin Ⅶ

the quality control indexes of medicinal materials in

Chinese Pharmacopoeia

(2015 edition) were detected in rhizomes of 8 species

namely

P

.

polyphylla

var.

yunnanensis

P

.

delavayi

P

.

mairei

P

.

polyphylla

var.

yunnanensis

(kuoban)

P

.

veitnamensis

P

.

axialis

P

.

thibetica

and

P

.

polyphylla

var.

polyphylla

polyphyllin Ⅴ was also detected in the study. There were significant differences in active ingredients of polyphyllin Ⅲ (Dioscin)

polyphyllin H and gracillin. Meanwhile

P

.

polyphylla

var.

yunnanensis

(S1)

P

.

delavayi

(S2)

P

.

mairei

(S3)

P

.

polyphylla

var.

yunnanensis

(kuoban) (S4)

P

.

veitnamensis

(S5)

P

.

axialis

(S6) and

P

.

polyphylla

var.

polyphylla

(S9) could be classified into one category according to the principal component analysis (PCA) model of the LC-MS data under the positive ion mode of

Paris

.

P

.

thibetica

(S7) and

P

.

forrestii

(S8) were clustered as two separate categories

with a longest range from

P

.

polyphylla

var.

yunnanensi

.

Conclusion:

2

There are little differences in active ingredients and the principal ingredients of steroid saponins in rhizome of

P

.

polyphylla

var.

yunnanensis

P

.

delavayi

P

.

mairei

P

.

polyphylla

var.

yunnanensis

P

.

veitnamensis

P

.

axialis

and

P

.

polyphylla

var.

polyphylla

. However

further research is required to determine whether these could substitute

P

.

polyphylla

var.

yunnanensis

. as an alternative medicine.