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1.北京中医药大学 中药学院,北京 102488
2.北京中医药大学 东直门医院,北京 100700
[第一作者] 李婷,在读硕士,从事药用植物与分子生药学研究,Tel:010-84738624,E-mail:Abby1230@163.com
*华国栋,教授,从事临床中药与中药质量研究,Tel:010-67689957,E-mail:zhaojhuagd@126.com
*刘春生,教授,从事药用植物与分子生药学研究,Tel:010-84738624,E-mail:max_liucs@263.net;
收稿日期:2019-09-16,
网络出版日期:2019-11-06,
纸质出版日期:2020-03-05
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李婷, 姜丹, 常晓茜, 等. 16种植物挥发油对柏子仁黄曲霉菌的抑制作用考察[J]. 中国实验方剂学杂志, 2020,26(5):132-136.
Ting LI, Dan JIANG, Xiao-xi CHANG, et al. Study on Inhibitory Effect of Volatile Oils in 16 Plants on
李婷, 姜丹, 常晓茜, 等. 16种植物挥发油对柏子仁黄曲霉菌的抑制作用考察[J]. 中国实验方剂学杂志, 2020,26(5):132-136. DOI: 10.13422/j.cnki.syfjx.20200447.
Ting LI, Dan JIANG, Xiao-xi CHANG, et al. Study on Inhibitory Effect of Volatile Oils in 16 Plants on
目的:
2
从16种植物(香茅、香叶、松节、山苍子、薄荷、干姜、丁香、姜黄、红花椒、肉桂、罗勒、迷迭香、青花椒、竹叶花椒、八角茴香、肉豆蔻)挥发油中筛选出对黄曲霉菌生长有较好抑制效果的植物挥发油。
方法:
2
采用平板培养法从柏子仁药材表面分离得到黄曲霉菌,利用水蒸气蒸馏法提取16种植物挥发油,采用滤纸片熏蒸后测定黄曲霉菌的菌落直径,对16种植物挥发油抑制黄曲霉菌生长的效果进行研究。
结果:
2
采用性状、显微及DNA条形码鉴定的方法从柏子仁药材上成功分离了黄曲霉菌,上述16种植物挥发油对黄曲霉菌的抑菌率分别为2.93%,0.05%,0.37%,76.07%,0.34%,0.15%,50.05%,8.51%,1.43%,58.20%,0.07%,2.60%,8.73%,100.00%,52.62%,0.07%。
结论:
2
16种植物挥发油对黄曲霉菌均有着不同程度的抑菌活性,其中竹叶花椒挥发油、山苍子挥发油、肉桂挥发油的抑菌效果较好,可为柏子仁生长储藏过程中防治黄曲霉菌的污染提供参考,并为植物挥发油作为中药仓储过程中的抑菌剂提供应用依据。
Objective:
2
To select volatile oils from 16 species of plants (
Cymbopogon citratus
Pelargonium graveolens
Pinus tabulieformis
Litsea cubeba
Mentha haplocalyx
Zingiber officinale
Syzygium aromaticum
Curcuma longa
Zanthoxylum bungeanum
Cinnamomum cassia
Ocimum basilicum
Rosmarinus officinalis
Zanthoxylum schinifolium
Zanthoxylum armatum
Illicium verum
Myristica fragrans
) that have good inhibitory effect on the growth of
Aspergillus flavus
.
Method:
2
Aspergillus flavus
was isolated from the surface of Platycladi Semen medicinal materials by plate culture method. The volatile oils of 16 plants were extracted by steam distillation. The colony diameter of
Aspergillus flavus
was determined by fumigation of filter paper
and the effect of volatile oils on the growth of
Aspergillus flavus
was systematically studied.
Result:
2
Aspergillus flavus
was successfully isolated from Platycladi Semen by means of morphological
microscopic and DNA barcoding identification methods
the bacteriostatic rates of the above 16 kinds of volatile oils against
Aspergillus flavus
were 2.93%
0.05%
0.37%
76.07%
0.34%
0.15%
50.05%
8.51%
1.43%
58.20%
0.07%
2.60%
8.73%
100.00%
52.62%
0.07%
respectively.
Conclusion:
2
The volatile oils of 16 plants all have different degrees of antibacterial activities for
Aspergillus flavus
and volatile oils of
Zanthoxylum armatum
Litsea cubeba
and
Cinnamomum cassia
have good inhibitory effect. This study can provide a theoretical basis for the prevention and control of
Aspergillus flavus
in the growth and storage of Platycladi Semen
and provide a basis for further research on plant volatile oil as bacteriostatic agents in the storage process of traditional Chinese medicine.
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戴向荣 . 肉桂油抑制黄曲霉作用研究 [D]. 合肥 : 安徽农业大学 , 2007 .
Y J LI , W J KONG , M H LI , et al . Litsea cubeba essential oil as the potential natural fumigant:inhibition of Aspergillus flavus and AFB 1 production in licorice [J]. Ind Crop Prod , 2016 , 80 : 186 - 193 .
G AKARCA . Composition and antibacterial effect on food borne pathogens of Hibiscus surrattensis L. calyces essential oil [J]. Ind Crop Prod , 2019 , 137 : 285 - 289 .
马捷 , 李梅 , 宁莉萍 , 等 . 竹叶花椒枝和叶精油化学成分及其对二磷酸腺苷诱导血小板聚集的影响研究 [J]. 天然产物研究与开发 , 2019 , doi: 10.16333/j.1001-6880.2019.9.010 http://dx.doi.org/10.16333/j.1001-6880.2019.9.010 .
王纪辉 , 侯娜 , 梁美 . 水分胁迫下竹叶花椒的生理响应 [J]. 江苏农业学报 , 2019 , 35 ( 3 ): 676 - 681 .
唐海龙 , 龚伟 , 王景燕 , 等 . 水肥处理对竹叶花椒土壤养分和微生物的影响 [J]. 浙江农林大学学报 , 2019 , 36 ( 2 ): 318 - 325 .
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