细胞膜色谱及质谱联用技术在中药活性成分筛选中的应用

王玉; 杨雪; 夏鹏飞; 杨蕊菁; 王玉霞; 杨飞霞; 樊秦; 武晓玉; 彭雪晶; 赵磊

doi:10.13422/j.cnki.syfjx.20200513

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细胞膜色谱及质谱联用技术在中药活性成分筛选中的应用

学术探讨 | 更新时间：2021-02-09

- 细胞膜色谱及质谱联用技术在中药活性成分筛选中的应用
- Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines
- 中国实验方剂学杂志 2020年26卷第8期页码：241-250
- 作者机构：
  
  1.甘肃中医药大学　药学院，兰州　 730000
  2.北京中医药大学　药学院，北京　 102488
  3.甘肃中医药大学　甘肃省高校中藏药化学与质量研究省级重点实验室，兰州　 730000
  4.甘肃省道地药材质量标准化技术研究与推广工程实验室，兰州　 730000
- 作者简介：
  
  [第一作者]　王玉，在读博士，从事中药药效物质基础和质量标准研究，E-mail：704338647@qq.com
  *赵磊，教授，博士生导师，从事中药药效物质基础和质量标准研究，Tel：0931-8762539，E-mail：zzyhx@gszy.edu.cn
- 基金信息：
  
  国家自然科学基金项目(81660577);甘肃省中医药管理局科研项目(G2KLP-2014-7);甘肃省道地药材质量标准化技术研究与推广工程实验室开放基金项目(ddyc-2016-04);甘肃省中医药管理局科研项目(G2K-2014-77)
- DOI：10.13422/j.cnki.syfjx.20200513
  中图分类号： R284.2; R289; R22; R2-031
- 收稿日期：2019-06-14，
  
  网络出版日期：2019-11-27，
  
  纸质出版日期：2020-04-20
- 稿件说明：
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王玉, 杨雪, 夏鹏飞, 等. 细胞膜色谱及质谱联用技术在中药活性成分筛选中的应用[J]. 中国实验方剂学杂志, 2020,26(8):241-250.

Yu WANG, Xue YANG, Peng-fei XIA, et al. Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 241-250.
王玉, 杨雪, 夏鹏飞, 等. 细胞膜色谱及质谱联用技术在中药活性成分筛选中的应用[J]. 中国实验方剂学杂志, 2020,26(8):241-250. DOI： 10.13422/j.cnki.syfjx.20200513.

Yu WANG, Xue YANG, Peng-fei XIA, et al. Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 241-250. DOI： 10.13422/j.cnki.syfjx.20200513.

摘要

由于中药作用的整体性、成分和作用机制的复杂性，化学成分(群)作为中药及其复方制剂发挥治疗作用的物质基础，因此，采用多种方法分离分析中药及其复方中的化学成分一直是中药治病防病研究中一个有待解决的关键重要问题。传统方法存在操作繁琐、耗时耗事、浪费溶剂和样品的不可逆吸附等缺陷，并且分离过程中无法给出药理参数的基本信息，因而无法对活性成分进行高效准确定位。近年来从细胞层次研究药物成分与受体的结合为中药活性成分的筛选研究提供了一种新的途径。目前研究二者相互作用的方法之一—细胞膜色谱法(CMC)，将现有色谱及质谱技术、细胞生物学与受体药理学结合起来，能够正确反映活性部位、活性成分与细胞膜及膜受体的相互作用，对复杂中药体系中有效部位筛选、活性成分的分离和高通量筛选具有独特优势。该文综述了CMC的原理、特点、中药活性成分筛选领域中的细胞膜模型及其与气相色谱-质谱(GC-MS)及液相色谱-质谱(LC-MS)联用的研究现状，并对其今后研究方法和未来的开发前景进行了探讨，为CMC在中医药领域的深入研究和开发利用提供理论依据和实践指导。

Abstract

The chemical components (groups) contained in traditional Chinese medicine(TCM) and its compound preparations are the material basis for its curative effect

because of the integrity of the action of TCM and the complexity of its compositions and mechanism. The separation and analysis of chemical constituents in TCM and its compound prescriptions by various methods is always a key problem to be solved in the research of disease prevention and treatment of TCM. The binding of drug molecules to receptors at the cellular level was explored to provide a new idea for the screening of active components of TCM or its compound preparations. Traditional methods have some drawbacks

such as cumbersome operation

time-consuming

waste of solvents and irreversible adsorption of samples. The basic information of pharmacological parameters cannot be given in the separation process

and the active ingredients cannot be efficiently and accurately located. At present

cell membrane chromatography (CMC)

one of the methods

is used to study the interaction between drug molecules and receptors

and can combine the existing chromatography and mass spectrometry technology

cell biology and receptor pharmacology

and correctly reflect the interaction between active parts

active components and cell membrane and membrane receptors

so it has unique advantages in screening effective parts

separation of active components and high-throughput screening from complex TCM system. The principles and characteristics of CMC

the cell membrane model in the field of active ingredient selection of TCM and its research status in combination with gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were reviewed

and its development prospects and future research methods were discussed

which provides theoretical basis and practical guidance for the research and utilization of CMC in the field of TCM.

关键词

Keywords

references

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