黑逍遥散对APP/PS1双转基因小鼠海马和脑皮层CaMKⅡ<italic style="font-style: italic">α</italic>蛋白及其磷酸化表达的影响

马春林; 吴红彦; 段永强; 施丽娟; 廖云立

doi:10.13422/j.cnki.syfjx.20200640

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黑逍遥散对APP/PS1双转基因小鼠海马和脑皮层CaMKⅡα蛋白及其磷酸化表达的影响

经典名方 | 更新时间：2021-02-09

- 黑逍遥散对APP/PS1双转基因小鼠海马和脑皮层CaMKⅡα蛋白及其磷酸化表达的影响
- Effect of Hei Xiaoyaosan on Expression of CaMKⅡα Protein and Its Phosphorylation in Hippocampus and Cerebral Cortex of APP/PS1 Double Transgenic Mice
- 中国实验方剂学杂志 2020年26卷第6期页码：25-31
- 作者机构：
  
  1.陕西中医药大学　第一临床医学院，陕西咸阳　 721046
  2.上海中医药大学　深圳医院，广东深圳　 518020
  3.甘肃中医药大学，兰州　 730000
- 作者简介：
  
  [第一作者]　马春林，博士，从事痴呆的中医药防治研究，E-mail：757695095@qq.com
  *吴红彦，教授，博士生导师，从事方证相关理论及敦煌医学传承与创新研究，E-mail：wu.hy@163.com
- 基金信息：
  
  国家自然科学基金项目(81660760)
- DOI：10.13422/j.cnki.syfjx.20200640
  中图分类号： R2-0; R289; R285.5
- 收稿日期：2019-09-12，
  
  网络出版日期：2019-12-09，
  
  纸质出版日期：2020-03-20
- 稿件说明：
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马春林, 吴红彦, 段永强, 等. 黑逍遥散对APP/PS1双转基因小鼠海马和脑皮层CaMKⅡα蛋白及其磷酸化表达的影响[J]. 中国实验方剂学杂志, 2020,26(6):25-31.

Chun-lin MA, Hong-yan WU, Yong-qiang DUAN, et al. Effect of Hei Xiaoyaosan on Expression of CaMKⅡα Protein and Its Phosphorylation in Hippocampus and Cerebral Cortex of APP/PS1 Double Transgenic Mice[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 25-31.
马春林, 吴红彦, 段永强, 等. 黑逍遥散对APP/PS1双转基因小鼠海马和脑皮层CaMKⅡα蛋白及其磷酸化表达的影响[J]. 中国实验方剂学杂志, 2020,26(6):25-31. DOI： 10.13422/j.cnki.syfjx.20200640.

Chun-lin MA, Hong-yan WU, Yong-qiang DUAN, et al. Effect of Hei Xiaoyaosan on Expression of CaMKⅡα Protein and Its Phosphorylation in Hippocampus and Cerebral Cortex of APP/PS1 Double Transgenic Mice[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 25-31. DOI： 10.13422/j.cnki.syfjx.20200640.

摘要

目的：

观察黑逍遥散对阿尔茨海默症(AD)小鼠海马和脑皮层钙调蛋白依赖性激酶2

(CaMKⅡ

)及其磷酸化表达水平的干预效应。

方法：

30只APP /PS1双转基因雄性小鼠称体质量并按随机原则分为模型组、盐酸多奈哌齐组、黑逍遥散组，每组10只。同月龄、同种系的野生型C57BL/6雄性小鼠10只为空白组。盐酸多奈哌齐组(6 g·kg

-1

)、黑逍遥散组(3.25 mg·kg

-1

)分别连续给药90 d后，Morris水迷宫检测行为学变化，免疫组化和蛋白免疫印迹法检测小鼠海马区和脑皮层CaMKⅡ

蛋白及其磷酸化的表达。

结果：

干预90 d后，与空白组比较，AD模型组小鼠，逃避潜伏期显著延长，跨原平台和有效区域次数显著减少(

0.01)，小鼠海马区及脑皮层CaMKⅡ

蛋白表达显著减弱或降低，而p-CaMKⅡ

蛋白表达显著升高(

0.05，

0.01)；与模型组比较，盐酸多奈哌齐和黑逍遥散组小鼠的逃避潜伏期均显著缩短、跨原平台次数均显著增加(

0.01)，小鼠海马区及脑皮层CaMKⅡ

蛋白显著增强或升高，p-CaMKⅡ

蛋白表达显著降低(

0.05，

0.01)。

结论：

黑逍遥散通过调节AD小鼠不同脑区参与细胞记忆形成机制的关键蛋白CaMKⅡ

及其磷酸化表达，进而发挥改善AD小鼠的学习记忆能力。

Abstract

Objective:

To study the effect of Hei Xiaoyaosan on the expression of calcium calmodulin-dependent protein kinase Ⅱ alpha(CaMKⅡ

) and its phosphorylation in hippocampus and cortex of mice with Alzheimer's disease.

Method:

After weighing

30 APP/PSI transgenic male mice were divided into model group

donepezil hydrochloride group and Hei Xiaoyaosan group according to random principle and 10 in each group.At the same age

wild-type C57BL/6 10 mice of the same species were treated as blank group. Donepezil hydrochloride group (6 g·kg

-1

) and Hei Xiaoyaosan group (3.25 mg·kg

-1

) were administered for 90 days

then the behavior of all the mice were detected by Morris water maze

the expression of CaMKⅡ

p-CaMKⅡ

proteins in hippocampus and cortex by immunohistochemical technique and Western blot.

Result:

After intervention 3 months

compared with blank group

the average escaping latency periods prolonged significantly and the number of cross-platform and effective areas were decreased distinctly in model group mice(

0.01)

CaMKⅡ

protein relative expression decreased significantly(

0.01)

p-CaMKⅡ

protein relative expression increased significantly(

0.01). Compared with the model group

the escape latency of donepezil hydrochloride and Hei Xiaoyaosan group were significantly shortened

and the number of crossing platforms and effective areas was significantly increased (

0.05

0.01)

the expression of CaMKⅡ

protein in the hippocampus and cortex of drug groups was significantly increased (

0.01)

p-CaMKⅡ

protein in the hippocampus and cortex of drug groups was significantly decreased (

0.05

0.01).

Conclusion:

Hei Xiaoyaosan can improve the learning and memory ability of AD mice by regulating the expression of CaMKⅡ

and its phosphorylation

which are key proteins involved in the mechanism of cell memory formation in different brain regions of AD mice.

关键词

Keywords

references

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黑逍遥散对APP/PS1双转基因小鼠海马和脑皮层CaMKⅡα蛋白及其磷酸化表达的影响

Effect of Hei Xiaoyaosan on Expression of CaMKⅡα Protein and Its Phosphorylation in Hippocampus and Cerebral Cortex of APP/PS1 Double Transgenic Mice

DOI：10.13422/j.cnki.syfjx.20200640

摘要

Abstract

关键词

Keywords

references