人参-三七-川芎提取物对糖尿病小鼠心肌纤维化的保护作用

胡艳红; 王雪; 方靖漪; 杨静; 修成奎; 王佳丽; 刘奕清; 雷燕

doi:10.13422/j.cnki.syfjx.20200703

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人参-三七-川芎提取物对糖尿病小鼠心肌纤维化的保护作用

益气活血药干预高糖诱导血管衰老的研究专题 | 更新时间：2021-02-09

- 人参-三七-川芎提取物对糖尿病小鼠心肌纤维化的保护作用
- Protective Effect of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma Extracts on Myocardial Fibrosis in Diabetic Mice
- 中国实验方剂学杂志 2020年26卷第8期页码：30-37
- 作者机构：
  
  1.中国中医科学院　医学实验中心，北京市中医药防治重大疾病基础研究重点实验室，北京　 100700
  2.广东药科大学　中医药研究院，广东省代谢性疾病中西医结合研究中心，广州　 510006
- 作者简介：
  
  [第一作者]　胡艳红，在读博士，从事中西医结合心血管病研究，Tel：010-64089525，E-mail：huyanhong3737@163.com
  *雷燕，博士，首席研究员，从事中西医结合心血管病研究，Tel：010-84076318，E-mail：13651217893@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81673822);中国中医科学院自主选题项目(ZZ2018014;ZZ2017011)
- DOI：10.13422/j.cnki.syfjx.20200703
  中图分类号： R2-0; R22; R285.5
- 收稿日期：2019-10-12，
  
  网络出版日期：2019-12-18，
  
  纸质出版日期：2020-04-20
- 稿件说明：
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胡艳红, 王雪, 方靖漪, 等. 人参-三七-川芎提取物对糖尿病小鼠心肌纤维化的保护作用[J]. 中国实验方剂学杂志, 2020,26(8):30-37.

Yan-hong HU, Xue WANG, Jing-yi FANG, et al. Protective Effect of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma Extracts on Myocardial Fibrosis in Diabetic Mice[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 30-37.
胡艳红, 王雪, 方靖漪, 等. 人参-三七-川芎提取物对糖尿病小鼠心肌纤维化的保护作用[J]. 中国实验方剂学杂志, 2020,26(8):30-37. DOI： 10.13422/j.cnki.syfjx.20200703.

Yan-hong HU, Xue WANG, Jing-yi FANG, et al. Protective Effect of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma Extracts on Myocardial Fibrosis in Diabetic Mice[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 30-37. DOI： 10.13422/j.cnki.syfjx.20200703.

摘要

目的：

从心肌纤维化程度及心肌组织中胶原蛋白Ⅰ型(Collagen Ⅰ)，胶原蛋白Ⅲ型(Collagen Ⅲ)及转化生长因子

(TGF-

)蛋白表达，探讨人参-三七-川芎提取物(GNC)对糖尿病小鼠心肌纤维化的保护作用。

方法：

建立链脲佐菌素(STZ)联合高脂饲料诱导糖尿病小鼠模型，另设正常组，糖尿病小鼠按照随机数字表法分为模型组，GNC低、高剂量组(0.819，1.638 g·kg

-1

)及二甲双胍组(150 mg·kg

-1

)。各组均灌胃给药，正常组给与等剂量的去离子水，1次/d，连续9周。胶原纤维染色(Masson三色染色法)观察小鼠心肌间质纤维化程度，并用Image-pro plus 6.0分析软件计算胶原密度比值；免疫组化检测小鼠心肌组织Collagen Ⅰ，Collagen Ⅲ及TGF-

蛋白表达情况；蛋白免疫印迹法(Western blot)检测小鼠心肌组织内Collagen Ⅰ，Collagen Ⅲ和TGF-

的蛋白表达电泳及灰度值水平。

结果：

Masson实验结果显示，与正常组比较，模型组小鼠心肌细胞肥大、变形，心肌间质尤其是在微血管周围，有大量蓝染的胶原纤维沉积，相互连接交织成网状(

0.01)；与模型组比较，GNC低、高剂量组和二甲双胍组小鼠心肌细胞排列紊乱明显改善，心肌间质蓝染的胶原纤维也较模型组明显减少(

0.05)。免疫组化和Western blot结果显示：与正常组比较，模型组小鼠心肌组织Collagen Ⅰ，Collagen Ⅲ和TGF-

阳性表达，蛋白表达含量明显升高(

0.05，

0.01)；与模型组比较，各给药组蛋白阳性表达均降低，蛋白表达含量趋于正常(

0.05，

0.01)。

结论：

STZ联合高脂饲料能够诱导糖尿病小鼠心肌纤维化，诱导Collagen Ⅰ，Collagen Ⅲ和TGF-

蛋白高表达。人参-三七-川芎提取物可通过调节Collagen Ⅰ，Collagen Ⅲ和TGF-

蛋白表达，改善糖尿病小鼠心肌纤维化。

Abstract

Objective:

To investigate the protective effect of Ginseng Radix et Rhizoma

Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma (GNC) extracts on myocardial fibrosis in diabetic mice by observing the degree of myocardial fibrosis and collagen types I (Collagen Ⅰ)

collagen types Ⅲ (Collagen Ⅲ) and transforming growth factor-

(TGF-

) protein expression in myocardial tissues.

Method:

A diabetic mice model was induced by streptozotocin (STZ) and high-fat diet. A normal control group was established. According to random number table method

diabetic mice were divided into model group

GNC low-dose and high-dose groups (0.819

1.638 g·kg

-1

)

and metformin group (150 mg·kg

-1

). Intragastrical administration was given in all groups

and the mice in normal control group received an equal dose of deionized water once a day for 9 weeks. The myocardial interstitial fibrosis in mice was observed by Masson trichromatic staining. Image-pro plus 6.0 analysis software was used to calculate the ratio of collagen area to total area. Immunohistochemistry was used to detect Collagen I

Collagen Ⅲ and TGF-

protein expression in myocardial tissues. The protein expression electrophoresis and gray value levels of Collagen I

Collagen Ⅲ and TGF-

in the myocardial tissues were detected by Western blot.

Result:

The results of Masson staining showed that as compared with the normal control group

the myocardial cells of diabetic mice were hypertrophic and disordered

and the myocardial stroma

especially the blue-stained collagenous fibers around the blood vessels

were heavily deposited and connected to each other in a network (

0.01). As compared with the model group

the arrangement of myocardial cells was significantly improved in GNC low-dose and high-dose groups and metformin group

and the collagenous fibers in the myocardial stroma were significantly decreased (

0.05). Immunohistochemistry and Western blot results showed positive expression of Collagen Ⅰ

Collagen Ⅲ and TGF-

in myocardial tissues

with significantly increased content of protein expression in diabetic mice (

0.05

0.01). As compared with the model group

the positive protein expression decreased and the protein content tended to be normal in each administration group (

0.05

0.01).

Conclusion:

High-fat diet combined with STZ can induce myocardial fibrosis in diabetic mice

and increase Collagen I

Collagen Ⅲ and TGF-

protein expression. Ginseng Radix et Rhizoma

Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma extracts can improve myocardial fibrosis in diabetic mice by regulating the expression of Collagen I

Collagen Ⅲ and TGF-

protein.

关键词

Keywords

references

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