鼻敏方通过NF-<italic style="font-style: italic">κ</italic>B信号通路对肺脾气虚变应性鼻炎大鼠鼻黏膜水通道蛋白5的影响

冯小聪; 黄唯; 周世卿; 谭希; 刘华娜; 陈海; 夏纪严; 李云英; 罗秋兰

doi:10.13422/j.cnki.syfjx.20200837

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鼻敏方通过NF-κB信号通路对肺脾气虚变应性鼻炎大鼠鼻黏膜水通道蛋白5的影响

药理 | 更新时间：2021-02-09

- 鼻敏方通过NF-κB信号通路对肺脾气虚变应性鼻炎大鼠鼻黏膜水通道蛋白5的影响
- Effect of Bimin Decoction on Aquaporin 5 in Nasal Mucosa of Allergic Rhinitis Rats with Lung and Spleen Qi Deficiency Syndrome Via NF-κB Signaling Pathway
- 中国实验方剂学杂志 2020年26卷第8期页码：81-88
- 作者机构：
  
  1.广州中医药大学　第二临床医学院，广州　 510405
  2.广州中医药大学　第二附属医院，广州　 510120
- 作者简介：
  
  [第一作者]　冯小聪，在读硕士，从事耳鼻喉科疾病中西医结合的临床与基础研究，E-mail：xcongzy@163.com
  *罗秋兰，博士，主治医师，从事耳鼻喉疾病中西医结合的临床与基础研究，E-mail：doctorluoql77@gzucm.edu.cn
- 基金信息：
  
  国家自然科学基金项目(81603667);国家中医药管理局国家中医临床研究基地业务建设科研专项(JDZX2015204);广东省中医院中医药科学技术研究专项(YN2015MS18;YN2016QL07);广东省中医药局科研项目(20192039)
- DOI：10.13422/j.cnki.syfjx.20200837
  中图分类号： R2-0; R289; R285.5; R765.21
- 收稿日期：2019-09-28，
  
  网络出版日期：2020-01-06，
  
  纸质出版日期：2020-04-20
- 稿件说明：
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冯小聪, 黄唯, 周世卿, 等. 鼻敏方通过NF-κB信号通路对肺脾气虚变应性鼻炎大鼠鼻黏膜水通道蛋白5的影响[J]. 中国实验方剂学杂志, 2020,26(8):81-88.

Xiao-cong FENG, Wei HUANG, Shi-qing ZHOU, et al. Effect of Bimin Decoction on Aquaporin 5 in Nasal Mucosa of Allergic Rhinitis Rats with Lung and Spleen Qi Deficiency Syndrome Via NF-κB Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 81-88.
冯小聪, 黄唯, 周世卿, 等. 鼻敏方通过NF-κB信号通路对肺脾气虚变应性鼻炎大鼠鼻黏膜水通道蛋白5的影响[J]. 中国实验方剂学杂志, 2020,26(8):81-88. DOI： 10.13422/j.cnki.syfjx.20200837.

Xiao-cong FENG, Wei HUANG, Shi-qing ZHOU, et al. Effect of Bimin Decoction on Aquaporin 5 in Nasal Mucosa of Allergic Rhinitis Rats with Lung and Spleen Qi Deficiency Syndrome Via NF-κB Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 81-88. DOI： 10.13422/j.cnki.syfjx.20200837.

摘要

目的：

观察鼻敏方对肺脾气虚变应性鼻炎(AR)大鼠核转录因子-

B(NF-

B)信号通路及水通道蛋白5(AQP5)表达的影响，探讨鼻敏方治疗AR的作用机制。

方法：

56只SD大鼠随机分7组：正常组，AR组，肺脾气虚AR组，鼻敏方低剂量2周组和4周组、鼻敏方高剂量2周组和4周组。正常组不干预，AR组以卵清蛋白(OVA)为致敏原建立AR疾病模型；其余5组大鼠除了以烟熏及番泻叶冷服泻下建立肺脾气虚证候模型外，还与AR组同期接受OVA致敏建立AR疾病模型。造模成功后鼻敏方组分别予低剂量鼻敏方(11.3 g·kg

-1

)干预2周和4周，高剂量鼻敏方(22.6 g·kg

-1

)干预2周和4周。观察大鼠的一般情况，苏木素-伊红(HE)染色观察鼻黏膜病理改变，免疫组化(IHC)和蛋白免疫印迹法(Western blot)检测NF-

B，AQP5蛋白的表达水平，实时荧光定量聚合酶链式反应(Real-time PCR)检测白细胞介素-1

(IL-1

)，肿瘤坏死因子-

(TNF-

)，白细胞介素-6(IL-6)，AQP5 mRNA表达水平。

结果：

AR组大鼠出现典型AR症状，肺脾气虚AR大鼠同时出现典型AR症状及肺脾气虚症状，鼻敏方干预后AR症状及肺脾气虚症状表现明显改善；与正常组比较，AR组及肺脾气虚AR组出现典型的鼻黏膜组织病理改变，行为学评分明显升高(

0.05)，NF-

B和AQP5蛋白表达上升(

0.05)，IL-1

，TNF-

，IL-6和AQP5 mRNA表达上升(

0.05)；与AR组比较，肺脾气虚AR组鼻黏膜组织病理改变更严重，胞核NF-

B蛋白表达上升(

0.05)，TNF-

，AQP5 mRNA表达上升(

0.05)；与肺脾气虚AR组比较，鼻敏方干预组鼻黏膜组织病理损伤改善，NF-

B和AQP5蛋白表达下降(

0.05)，IL-1

，TNF-

，IL-6和AQP5 mRNA表达下降(

0.05)；与鼻敏方低剂量2周组比较，鼻敏方高剂量4周组胞核NF-

B蛋白表达下降(

0.05)。

结论：

与AR大鼠比较，在相同致敏原刺激下肺脾气虚AR大鼠病情更严重；鼻敏方可治疗AR并能减轻腺体过度分泌，其机制可能与抑制NF-

B信号通路，进而降低AQP5表达有关。

Abstract

Objective:

To observe the effect of Bimin decoction(BMD) on nuclear factor-kappa B(NF-

B) signaling pathway and aquaporin 5(AQP5) expression in allergic rhinitis (AR) rats with lung and spleen Qi deficiency syndrome(LSQDS)

in order to study the mechanism in treating AR.

Method:

Fifty-six SD rats were randomly divided into seven groups: control group

AR group

LSQDS AR group

BMD low dose two weeks group and four weeks group

BMD high dose two weeks group and four weeks group. The control group did not intervened

the AR group established the AR disease model with ovalbumin (OVA) as the allergen

the other five groups established the LSDQS model with smoke and senna gavage

and also established the AR disease model with OVA sensitization at the same time as the AR group. After the model was established successfully

four BMD intervention groups were separately given low dose BMD (11.3 g·kg

-1

) for 2 weeks and 4 weeks

and high dose BMD (22.6 g·kg

-1

) for 2 weeks and 4 weeks. To observe the general situation of the rats

hematoxylin eosin (HE) staining was used to observe the pathological changes of the nasal mucosa

immunohistochemistry and Western blot were used to detect the expression levels of NF-

B and AQP5

real-time fluorescent quantitative polymerase chain reaction technique (Real-time PCR)was used to detect mRNA levels of interleukin-1

(IL-1

)

tumor necrosis factor-

(TNF-

)

interleukin-6 (IL-6).

Result:

The typical AR symptoms were found in AR rats

the AR symptoms and lung and spleen Qi deficiency symptoms were found in AR rats with LSQDS at the same time

and the AR symptoms and lung and spleen Qi deficiency symptoms were significantly improved after the intervention of BMD. Compared with the control group

the typical histopathological changes of nasal mucosa were found in AR group and LSQDS AR group

with a higher behavioral score (

0.05)

and the expression of NF-

B and AQP5 protein increased (

0.05)

the expression of IL-1

TNF-

IL-6 and AQP5 mRNA increased (

0.05). Compared with AR group

the pathological changes of nasal mucosa in LSQDS AR group were more serious

and the expression of NF-

B protein in nucleus increased (

0.05)

the expression of TNF-

and AQP5 mRNA increased (

0.05). Compared with LSQDS AR group

the pathological changes of nasal mucosa in the groups which interfered by BMD were improved

and the expression of NF-

B and AQP5 protein decreased (

0.05)

the expression of IL-1

TNF-

IL-6 and AQP5 mRNA decreased (

0.05). Compared with BMD low-dose two-week group

the expression of NF-

B protein in nucleus decreased (

0.05) in BMD high dose four week group.

Conclusion:

Compared with AR group

the AR condition of the rats with LSQDS is more serious under the same allergen stimulation

BMD can treat AR and reduce the over secretion of glands

which may be related to inhibit the expression of AQP5 by inhibiting the NF-

B signaling pathway.

关键词

Keywords

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Effect of Bimin Decoction on Aquaporin 5 in Nasal Mucosa of Allergic Rhinitis Rats with Lung and Spleen Qi Deficiency Syndrome Via NF-κB Signaling Pathway

DOI：10.13422/j.cnki.syfjx.20200837

摘要

Abstract

关键词

Keywords

references