Objective:

2

To study the effect of total alkaloids of

Nandina domestica

in attenuating arsenic trioxide

and analyze chemical constituents of alkaloids extract of

Nandina

in order to provide the theoretical basis for studying the effect of

N

.

domestica

in attenuating arsenic trioxide with alkaloids extract of

N

.

domestica

as effective fraction.

Method:

2

The model of acute liver injury induced by arsenic trioxide was used to compare the effects on heart and liver functions of mice between arsenic trioxide alone and total alkaloids of

N

.

domestica

combined with arsenic trioxide. The detoxification of total alkaloids on arsenic trioxide was evaluated based on biochemical parameters and pathological report. Peakview (Version1.2

AB SCIEX) software was used to process the mass spectrometry data of total alkaloids of

N

.

domestica

. The structures of determined compounds were identified by molecular weight of compound (molecular formula)

secondary fragments

chromatographic peak retention and literature information.

Result:

2

Among biochemical indicators

creatine kinase(CK)

lactate dehydrogenase(LDH)

blood urea nitrogen(BUN)

malondialdehyde(MDA)

alanine aminotransferase(ALT) and amino transferase of aspartate(AST) of the arsenic trioxide group were increased

while elimination rates of Na

+

-K

+

-adenosine triphosphate(ATP)

superoxide dismutase(SOD)

catalase(CAT) and serum creatinine(SCr) were decreased

compared with those of the combination group. CK and LDH of the alkaloids extract group were more obviously increased than those of the

N

.

domestica

extract group

but with no remarkable difference. In histomorphometric examination

edema of mouse heart cells was improved

and some kidney and liver damages in rats were alleviated. Totally 25 alkaloids of alkaloid extract were identified. Among them

18 were known

and 7 were unknown

including 3 structural types

in which apomorphine alkaloids were mostly.

Conclusion:

2

Heart

kidney and liver damage degrees of the combination group were significantly alleviated compared with the arsenic trioxide group. The total alkaloids fraction extracted and purified have a significant attenuation in arsenic trioxide toxicity. The detoxification of total alkaloids extract was equal to that of

N

.

domestica

extract. Furthermore

apomorphine alkaloids

such as nantenine and domesticine

can be used as index components to establish a quality control method for total alkaloids.