Objective:

2

The processing method of red ginseng was determined by comparing the effects of different steaming time and pressure on the total content of six ginsenosides.

Method:

2

The contents of ginsenoside Rg

1

Re

Rf

Rb

1

Rc and Rb

2

were determined by ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS/MS). The Waters ACQUITY UPLC BEH C

8

column (2.1 mm×100 mm

1.7 μm) was used. The mobile phase was 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile solution (B) for gradient elution (0-4 min

81%-79%A; 4-6.3 min

79%-75%A; 6.3-6.5 min

75%-71%A; 6.5-9.5 min

71%A; 9.5-16.5 min

71%-68.5%A; 16.5-16.6 min

68.5%-60%A; 16.6-19 min

60%-100%A). The flow rate was set at 0.4 mL·min

-1

and the column temperature was set at 35 ℃. The mass spectrographic analysis employed electrospray ionization (ESI) and negative ion collection mode with capillary ionization voltage of 2.5 kV

desolvation temperature of 350 ℃

desolvation gas flow of 700 L·h

-1

and cone gas flow of 50 L·h

-1

. Multiple reaction monitoring (MRM) mode was used to collect information

the collection range was

m

/

z

100-1 500

detection was performed by MRM mode at

m

/

z

799.59-637.49 for ginsenoside Rg

1

m

/

z

945.54-475.79 for ginsenoside Re

m

/

z

799.59-475.49 for ginsenoside Rf

m

/

z

1 107.59-783.97 for ginsenoside Rb

1

m

/

z

1 077.58-783.96 for ginsenoside Rc

m

/

z

1 077.75-191.19 for ginsenoside Rb

2

.

Result:

2

When the steaming time was 3 hours

the total mass fraction of six ginsenosides in each sample group was 7.099 8-16.768 5 mg·g

-1

and the total amount of the six ginsenosides in atmospheric steaming was 2.5-12.6 times of that in pressurized steaming

which was obviously better than that in pressurized steaming.

Conclusion:

2

Under the conditions of this experiment

the best processing method of red ginseng is atmospheric steaming for 3 hours with fresh ginseng.