Objective

2

To observe the expression of brown adipose tissue (BAT)

cells

proteins and corresponding genes in Yang deficiency model mice induced by Rhei Radix et Rhizoma suspension

and to explore the thermogenesis of processed products of Aconiti Lateralis Radix Praeparata with Jianchang faction characteristics.

Method

2

Twenty mice

half male and half female

were randomly selected as the normal female and male groups. And the other 80 mice were administrated with Rhei Radix et Rhizoma suspension (the content of 0.25 g·mL

-1

) to establish Yang deficiency model

after the model was established

they were randomly divided into the model female and male groups

female and male groups of Shengfupian

female and male groups of Yinfupian

female and male groups of Yangfupian

10 mice in each group. Mice were intragastric administrated with corresponding medical solution for two weeks (1.54 g·kg

-1

·d

-1

) according to groups. Normal group and model group were given equal volume distilled water. After administration

BAT of scapular region of mice was collected and the changes of BAT cells were observed by hematoxylin-eosin (HE) staining. The expression of uncoupling protein 1 (UCP1) and its mRNA were detected by Western blot and real-time fluorescence quantitative polymerase chain reaction (Real-time PCR).

Result

2

Compared with the normal group of the same sex

the proportion of BAT in the model group decreased significantly (

P

<

0.01). Compared with the model group of the same sex

the proportion of BAT in female mice from Shengfupian and Yinfupian groups increased significantly (

P

<

0.01)

while there was no significant difference between each administration group and model group in the male mice. Compared with normal mice of the same sex

there were many scattered vacuoles in BAT cells of the model group

and fewer cells could be observed due to larger vacuoles. Compared with the model group of the same sex

BAT cells in mice from the Shengfupian group showed fewer vacuoles

smaller cells and tight arrangement

the density of BAT cells in mice from the Yangfupian group also increased significantly

while the vacuoles in BAT cells of mice from the Yinfupian group decreased relatively and the cells did not increase significantly. Compared with the same sex mice

the expression level of UCP1 in the model group and the normal group was statistically significant (

P

<

0.05

P

<

0.01). In the female mice

the expression level of UCP1 in Yangfupian group was significantly higher than that in the model group (

P

<

0.05)

each administration group of male mice was significantly different from that of the model group of the same sex (

P

<

0.05)

of which Yangfupian was the most significant. The relative expression of UCP1 mRNA in the model group was significantly lower than that in the normal group of the same sex (

P

<

0.05

P

<

0.01). In the female mice

compared with the model group

the relative expression levels of UCP1 mRNA in Yangfupian group

Shengfupian group and Yinfupian group increased significantly (

P

<

0.05

P

<

0.01)

compared with Yangfupian group

the relative expression levels of UCP1 mRNA in Shengfupian and Yinfupian were also significantly different (

P

<

0.05). In the male mice

compared with the model group

the relative expression of UCP1 mRNA in Yangfupian group was significantly increased (

P

<

0.01)

but there was no significant difference in Shengfupian group and Yinfupian group

in addition

compared with Yangfupian group

the relative expression of UCP1 mRNA in Shengfupian group and Yinfupian group had significant difference (

P

<

0.05).

Conclusion

2

Shengfupian

Yinfupian and Yangfupian all have obvious improvement on Yang deficiency syndrome induced by Rhei Radix et Rhizoma suspension. The mechanism may be to promote the expression of UCP1 protein and its mRNA and enhance the activity of BAT. And the effect of Yangfupian is the best.