人参皂苷Rg<sub>1</sub>对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响

高月; 黄文祥; 章述军; 李佳俊; 阳成; 赵金秋; 肖晴; 杨福伟; 朱雅莉

doi:10.13422/j.cnki.syfjx.20201101

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人参皂苷Rg₁对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响

药理 | 更新时间：2020-08-11

- 人参皂苷Rg₁对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响
- Effect of Ginsenoside Rg₁ on Lipid Uptake and Oxidation in HepG2 Cells of Nonalcoholic Fatty Liver Disease
- 中国实验方剂学杂志 2020年26卷第12期页码：100-106
- 作者机构：
  
  重庆医科大学附属第一医院，重庆 400016
- 作者简介：
  
  高月，在读硕士，从事非酒精性脂肪性肝病研究，E-mail：yue_gaoo@163.com
  黄文祥，硕士，教授，从事感染性疾病、非酒精性脂肪性肝病研究，E-mail：wenxiang_huang@163.com
- 基金信息：
  
  重庆市科委基础科学与前沿技术研究重点项目(CSTC2015jcyjBX0079)
- DOI：10.13422/j.cnki.syfjx.20201101
  中图分类号： R2-0;R22;R285.5
- 网络出版日期：2020-03-03，
  
  纸质出版日期：2020-06-20
- 稿件说明：
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高月,黄文祥,章述军等.人参皂苷Rg₁对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响[J].中国实验方剂学杂志,2020,26(12):100-106.

GAO Yue,HUANG Wen-xiang,ZHANG Shu-jun,et al.Effect of Ginsenoside Rg₁ on Lipid Uptake and Oxidation in HepG2 Cells of Nonalcoholic Fatty Liver Disease[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):100-106.
高月,黄文祥,章述军等.人参皂苷Rg₁对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响[J].中国实验方剂学杂志,2020,26(12):100-106. DOI： 10.13422/j.cnki.syfjx.20201101.

GAO Yue,HUANG Wen-xiang,ZHANG Shu-jun,et al.Effect of Ginsenoside Rg₁ on Lipid Uptake and Oxidation in HepG2 Cells of Nonalcoholic Fatty Liver Disease[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):100-106. DOI： 10.13422/j.cnki.syfjx.20201101.

摘要

目的

探讨人参皂苷Rg

改善游离脂肪酸(FFA)诱导的非酒精性脂肪肝(NAFLD)HepG2细胞脂质摄取和氧化的作用及机制。

方法

将HepG2细胞分为空白组，模型组，人参皂苷Rg

低、高剂量组(25，50 μmol·L

-1

)。1 mmol·L

-1

游离脂肪酸处理HepG2细胞24 h构建非酒精性脂肪肝细胞模型，25，50 μmol·L

-1

人参皂苷Rg

处理24 h。细胞增殖及毒性检测-8（CCK-8）检测人参皂苷Rg

对HepG2细胞活性的影响，微量法检测细胞内甘油三酯（TG）含量，油红O染色观察脂滴聚集情况，实时荧光定量聚合酶链式反应（Real-time PCR）及蛋白免疫印迹法（Western blot）检测与脂质摄取和氧化相关基因与蛋白的表达情况。

结果

与空白组比较，模型组细胞内TG含量及脂滴聚集吸光度显著增加（

0.01）；与模型组比较，人参皂苷Rg

组细胞内TG含量及脂滴聚集吸光度显著降低（

0.01）。Real-time PCR和Western blot结果均显示，与空白组比较，模型组过氧化物酶体增殖物激活受体

(PPAR

)，脂肪酸结合蛋白1（FABP1），脂肪酸转运蛋白2/5（FATP2/5）和脂肪酸转运酶（CD36）mRNA和蛋白的表达明显升高（

0.05），过氧化物酶体增殖物激活受体

（PPAR

），肉毒碱棕榈酰基转移酶1（CPT1）和酰基辅酶A氧化酶1（ACOX1）mRNA和蛋白的表达明显降低（

0.05）；与模型组比较，人参皂苷Rg

组PPAR

，FABP1，FATP2，FATP5和CD36 mRNA和蛋白的表达明显降低（

0.05，

0.01），PPAR

，CPT1和ACOX1 mRNA和蛋白的表达明显升高（

0.05，

0.01）

。

结论

人参皂苷Rg

可通过降低脂质摄取和增强脂质氧化减少NAFLD细胞模型脂质蓄积。

Abstract

Objective

To investigate the effect and mechanism of ginsenoside Rg

（G-Rg

）in ameliorating lipid uptake and oxidation in HepG2 cells induced by free fatty acids (FFA).

Method

HepG2 cells were divided into normal group

model group，low-dose ginsenoside Rg

group (25 μmol·L

-1

) and high-dose G-Rg

group (50 μmol·L

-1

). HepG2 cells were treated with 1 mmol·L

-1

free fatty acid for 24 h to construct the NAFLD cell model

and then treated with 25，50 μmol·L

-1

G-Rg

for 24 h. The effect of G-Rg

on HepG2 cell activity was determined by cell counting kit-8（CCK-8） assay. The level of triglyceride (TG) was detected by micro method. The accumulation of lipid droplets was observed by oil red O staining. Quantitative real-time fluorescence polymerase chain reaction (Real-time PCR) and Western blot were used to detect the alterations of key genes and proteins relating to lipid uptake and metabolism.

Result

Compared with the normal group

the intracellular TG level and the absorbance of the oil red O staining in the model group were significantly increased (

0.01). Compared with the model group

G-Rg

reduced TG and lipid deposition were significantly reduced (

0.01).Results of Real-time PCR and Western blot showed that compared with normal group

model group peroxisome proliferators-activated receptors gamma（PPAR

），fatty acid binding protein 1（FABP1），fatty acid transport protein 2/5（FATP2/5）and fatty acid translocase（CD36）expressions increased（

0.05），whereas peroxisome proliferators-activated receptors

（PPAR

），carnitine palmitoyltransferase 1（CPT1）and peroxisomal acyl-coenzyme A oxidase 1（ACOX1）expressions decreased（

0.05）. Compared with the model group

the expressions of PPAR

FABP1

FATP2

FATP5 and CD36 in the G-Rg

group were decreased (

0.05，

0.01)

while the expressions of PPAR

CPT1 and ACOX1 were increased (

0.05，

0.01).

Conclusion

G-Rg

can ameliorate lipid deposition in NAFLD cell model by reducing lipid uptake and increasing lipid oxidation.

关键词

Keywords

references

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人参皂苷Rg1对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响

Effect of Ginsenoside Rg1 on Lipid Uptake and Oxidation in HepG2 Cells of Nonalcoholic Fatty Liver Disease

DOI：10.13422/j.cnki.syfjx.20201101

摘要

Abstract

关键词

Keywords

references

人参皂苷Rg₁对非酒精性脂肪肝HepG2细胞脂质摄取和氧化的影响

Effect of Ginsenoside Rg₁ on Lipid Uptake and Oxidation in HepG2 Cells of Nonalcoholic Fatty Liver Disease