血府逐瘀汤与加味二陈汤对小鼠非酒精性脂肪肝的作用比较

郭宏雅; 付远飞; 刘惠婷; 王剑

doi:10.13422/j.cnki.syfjx.20201103

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血府逐瘀汤与加味二陈汤对小鼠非酒精性脂肪肝的作用比较

经典名方 | 更新时间：2020-08-11

- 血府逐瘀汤与加味二陈汤对小鼠非酒精性脂肪肝的作用比较
- Comparasion on Effect of Xuefu Zhuyutang and Modified Erchentang on Nonalcoholic Fatty Liver in Mice
- 中国实验方剂学杂志 2020年26卷第12期页码：71-77
- 作者机构：
  
  1.广州中医药大学基础医学院，广州 510006
  2.广州中医药大学中西医结合基础研究中心，广州 510006
- 作者简介：
  
  郭宏雅，在读硕士，从事中医药防治心血管病的研究，E-mail:986502321@qq.com
  王剑，博士，教授，从事中医药延缓衰老的研究，E-mail：zswj@gzucm.edu.cn
- 基金信息：
  
  国家自然科学基金项目(8187151662)
- DOI：10.13422/j.cnki.syfjx.20201103
  中图分类号： R2-0;R22;R285.5;R289
- 网络出版日期：2020-03-04，
  
  纸质出版日期：2020-06-20
- 稿件说明：
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郭宏雅,付远飞,刘惠婷等.血府逐瘀汤与加味二陈汤对小鼠非酒精性脂肪肝的作用比较[J].中国实验方剂学杂志,2020,26(12):71-77.

GUO Hong-ya,FU Yuan-fei,LIU Hui-ting,et al.Comparasion on Effect of Xuefu Zhuyutang and Modified Erchentang on Nonalcoholic Fatty Liver in Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):71-77.
郭宏雅,付远飞,刘惠婷等.血府逐瘀汤与加味二陈汤对小鼠非酒精性脂肪肝的作用比较[J].中国实验方剂学杂志,2020,26(12):71-77. DOI： 10.13422/j.cnki.syfjx.20201103.

GUO Hong-ya,FU Yuan-fei,LIU Hui-ting,et al.Comparasion on Effect of Xuefu Zhuyutang and Modified Erchentang on Nonalcoholic Fatty Liver in Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):71-77. DOI： 10.13422/j.cnki.syfjx.20201103.

摘要

目的

观察比较血府逐瘀汤和加味二陈汤对高脂饮食诱导的载脂蛋白E敲除（ApoE

-/-

）小鼠非酒精性脂肪肝（NAFLD）的改善作用及机制。

方法

10只C57/BL6J小鼠为正常组，以一般饲料喂养，30只ApoE

-/-

小鼠为造模组，以高脂饲料喂养4周，后分为模型组、血府逐瘀汤组（以下简称为活血组）和加味二陈汤组（以下简称为化痰组），每组10只。正常组及模型组灌胃生理盐水，给药组以成人剂量的10倍予以灌胃，每天1次，共8周，于12周实验结束后取小鼠血清和肝脏。蛋白免疫印迹法（Western blot）与实时荧光定量聚合酶链式反应（Real-time PCR）测定各组血清血脂和肝功水平，观察肝脏病理形态，检测肝脏炎症介质白细胞介素-1

（IL-1

），肿瘤坏死因子-

（TNF-

），基质金属蛋白酶-9（MMP-9），单核细胞趋化因子-1（MCP-1）蛋白和mRNA表达水平。

结果

血清血脂与肝功结果显示，与正常组比较，模型组小鼠血清总胆固醇（TC），甘油三酯（TG），低密度脂蛋白（LDL），天门冬氨酸氨基转移酶（AST），丙氨酸氨基转移酶（ALT）显著升高，高密度脂蛋白（HDL）显著降低

（P

0.01）；与模型组比较，活血组小鼠血清TG，LDL

ALT明显降低，HDL明显升高

（P

0.05）；化痰组小鼠血清TC，TG，LDL，AST，ALT明显降低，HDL明显升高

（P

0.05，

0.01），其中化痰组小鼠血清HDL升高幅度较活血组高，活血组小鼠血清ALT降低幅度较化痰组大。病理学观察显示，与正常组比较，模型组肝细胞脂肪变性严重，存在许多脂滴空泡，提示小鼠NAFLD模型成功；与模型组比较，各给药组均减轻肝细胞脂肪性变

两给药组间并无显著差异。Western blot与Real-time PCR结果显示，与正常组比较，模型组TNF-

，IL-1

，MCP-1，MMP-9的蛋白与mRNA表达水平明显升高（

0.05，

0.01）；与模型组比较，活血组可明显下调IL-1

，MCP-1蛋白和MCP-1 mRNA的表达

（P

0.05，

0.01），化痰组可明显降低IL-1

，TNF-

，MMP-9，MCP-1蛋白和TNF-

，MMP-9 mRNA的表达（

0.05，

0.01）。

结论

加味二陈汤和血府逐瘀汤均可在一定程度上对NAFLD小鼠起到治疗改善的作用，其中加味二陈汤的治疗效果更佳。

Abstract

Objective

To compare the effect and mechanisms of modified Erchentang and Xuefu Zhuyutang on high-fat diet-induced apolipoprotein-E knockout (ApoE

-/-

) mice nonalcoholic fatty liver disease (NAFLD).

Method

Ten C57/BL6J mice were taken as normal control group and fed with normal feed. Totally 30 ApoE

-/-

mice were fed with high-fat diet to establish a disease model for 4 weeks. After 4 weeks

the 30 ApoE

-/-

mice were divided into model group

Xuefu Zhuyutang group (hereinafter referred to as Huoxue group) and modified Erchentang group (hereinafter referred to as Huatan group) by random number table method

with 10 in each group. The normal group and the model group were intragastrically administered with normal saline. The drug-administered group was intragastrically administered at a dosage that was ten times of the adult dose

once a day

for 8 weeks. Serum and liver were collected after the end of the 12-week experiment. The serum lipid and liver function levels of each group were measured

and the liver pathological morphology was observed. Protein and mRNA expressions of liver inflammatory mediators interleukin-1

(IL-1

)

tumor necrosis factor-

(TNF-

)

matrix metalloproteinase-9 (MMP-9)

monocyte chemotactic factor-1 (MCP-1) were detected by Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) and Western blot.

Result

The results of serum lipids and liver function showed that compared with the normal group

serum total cholesterol (TC)

triglyceride (TG)

low-density lipoprotein (LDL)

aspartate aminotransferase (AST)

and alanine aminotransferase (ALT) in the model group were significantly increased

while serum high-density lipoprotein (HDL) was significantly reduced (

0.01). Compared with the model group

serum TG ，LDL and ALT were significantly decreased

HDL was significantly increased in the Huoxue group (

0.05). The serum levels of TC

TG， LDL

AST and ALT in the Huatan group were significantly decreased，HDL was significantly increased (

0.05，

0.01)

and TG was decreased. The mice serum HDL in the Huatan group was higher than that in the Huoxue group. The serum ALT in the Huoxue group was lower than that in the Huatan group. The pathological observation showed that compared with the normal group

hepatocytes in the model group had severe steatosis with many lipid droplet vacuoles

suggesting that the mouse NAFLD model was successful. Compared with the model group

each administration group alleviated hepatocyte steatosis

with no significant difference between the two administration groups. Western blot and Real-time PCR results showed that compared with the normal group

protein and mRNA expression levels of TNF-

IL-1

MCP-1

and MMP-9 in the model group were significantly increased (

0.05，

0.01). Compared with the model group

the Huoxue group significantly down-regulated the expressions of IL-1

MCP-1 protein and MCP-1 mRNA(

0.05，

0.01). The Huatan group significantly reduced the expressions of IL-1

TNF-

MMP-9

MCP-1 protein

TNF-

and MMP-9 mRNA(

0.05，

0.01).

Conclusion

Modified Erchentang and Xuefu Zhuyutang can alleviate the therapeutic effect of NAFLD mice to a certain extent

modified Erchentang has a better therapeutic effect.

关键词

Keywords

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