温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3<italic style="font-style: italic">β</italic>表达的影响

田真真; 朱丽娟; 万红娇; 周步高; 徐义勇

doi:10.13422/j.cnki.syfjx.20201304

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温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3β表达的影响

经典名方 | 更新时间：2020-08-11

- 温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3β表达的影响
- Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment
- 中国实验方剂学杂志 2020年26卷第13期页码：45-51
- 作者机构：
  
  1.江西中医药大学，南昌 330004
  2.江西省中西医结合医院，南昌 330003
- 作者简介：
  
  田真真，在读博士，讲师，从事中医基础理论与诊治规律研究，E-mail：27939115@qq.com
  徐义勇，博士，副教授，从事中医基础理论与诊治规律研究，E-mail：xuyiyong2007@126.com
- 基金信息：
  
  国家自然科学基金项目(81860814);江西省自然科学基金项目(20192BAB205096);江西省教育厅科技计划项目(GJJ170741);江西省卫计委中医药科研课题(2017A235);江西中医药大学博士科研启动基金课题(2018WBZR020)
- DOI：10.13422/j.cnki.syfjx.20201304
  中图分类号： R2-0;R22;R285.5;R289
- 网络出版日期：2020-04-02，
  
  纸质出版日期：2020-07-05
- 稿件说明：
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田真真,朱丽娟,万红娇等.温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3β表达的影响[J].中国实验方剂学杂志,2020,26(13):45-51.

TIAN Zhen-zhen,ZHU Li-juan,WAN Hong-jiao,et al.Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):45-51.
田真真,朱丽娟,万红娇等.温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3β表达的影响[J].中国实验方剂学杂志,2020,26(13):45-51. DOI： 10.13422/j.cnki.syfjx.20201304.

TIAN Zhen-zhen,ZHU Li-juan,WAN Hong-jiao,et al.Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):45-51. DOI： 10.13422/j.cnki.syfjx.20201304.

摘要

目的

探讨温胆汤含药血清对谷氨酸环境下星型胶质细胞的保护作用及磷脂酰肌醇-3激酶（PI3K）/蛋白激酶B（Akt）/糖原合成酶激酶3

（GSK3

）信号通路的影响。

方法

将60只大鼠随机分为5组

其中正常组20只，氯氮平组、温胆汤高、中、低剂量组，每组10只。正常组给予20 mL·kg

-1

生理盐水灌胃，氯氮平组给予氯氮平原药20 mg·kg

-1

灌胃，温胆汤组分别给予剂量为40，20，10 g·kg

-1

的温胆汤灌胃，1次/d，8 d后处死大鼠，取血，离心取血清，灭活，过滤除菌，离心管分装备用。将星型胶质细胞分为空白组、模型组、氯氮平组、温胆汤高、中、低剂量组，其中空白组、模型组给予空白血清培养，其余各组给予相应含药血清培养；除空白组外，其余各组用10 mmol·L

-1

谷氨酸处理24 h后予流式细胞仪检测星形胶质细胞凋亡率；蛋白免疫印迹法（Western blot），实时荧光定量聚合酶链式反应（Real-time PCR）分别检测星型胶质细胞PI3K，Akt，GSK3

蛋白和mRNA的表达。

结果

与空白组比较，模型组细胞凋亡率显著升高（

0.01）；与模型组比较，温胆汤各剂量组细胞凋亡率则显著下降（

0.01）。与空白组比较，模型组细胞PI3K，Akt，GSK3

蛋白、磷酸化蛋白表达显著降低（

0.01）；与模型组比较，温胆汤各剂量组细胞PI3K，Akt，GSK3

蛋白、磷酸化蛋白表达明显升高（

0.05，

0.01）。与空白组比较，模型组细胞PI3K，Akt，GSK3

mRNA的表达显著降低（

0.01）；与模型组比较，温胆汤各剂量组细胞PI3K，Akt，GSK3

mRNA表达明显升高（

0.05，

0.01）。

结论

温胆汤含药血清可有效升高PI3K，Akt，GSK3

表达，从而调控PI3K/Akt/GSK3

信号通路，保护神经细胞。

Abstract

Objective

To investigate the protective effect of Wendantang-containing serum on astrocytes in glutamate environment and its effect on phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt)/glycogen synthetase kinase 3

(GSK3

) signal pathway.

Method

Totally 60 rats were randomly divided into 5 groups

normal group (

=20)

clozapine group

and high

medium and low-dose Wendantang groups

with 10 rats in each group. Normal group was given 20 mL·kg

-1

normal saline

clozapine group was given 20 mg·kg

-1

clozapine

Wendantang groups were given 40

10 g·kg

-1

Wendantang

once a day. Eight days later

the rats were killed

their blood was taken

serum was centrifuged

inactivated

filtrated

sterilized and filled in separate centrifugal tubes. The astrocytes were divided into normal group

model group

clozapine group

and high

medium and low-dose Wendantang groups. The normal group and the model group were cultured with normal serum

and the rest groups were cultured with corresponding drug containing serum. The other groups were treated with 10 mmol·L

-1

glutamic acid for 24 hours

and then the apoptosis of astrocytes was detected by flow cytometry. Western blot and Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR) were used to determine protein

and mRNA expressions of PI3K

Akt

GSK3

in astrocytes.

Result

Compared with the normal group

apoptosis in model group increased significantly (

0.01). Compared with the model group

apoptosis in Wendantang groups decreased significantly (

0.01). Compared with the normal group

protein and phosphorylation expressions of PI3K

Akt

GSK3

in model groups decreased significantly (

0.05

0.01). Compared with the model group

protein and phosphorylation expressions of PI3K

Akt

GSK3

in Wendantang groups increased (

0.05

0.01). Compared with the normal group

expressions of PI3K

Akt and GSK3

mRNA decreased in model group(

0.01). Compared with the model group

expressions of PI3K

Akt and GSK3

mRNA increased significantly in Wendantang groups group (

0.05，

0.01).

Conclusion

Wendantang-containing serum can effectively increase expressions of PI3K

Akt and GSK3

so as to regulate PI3K/Akt/GSK3

signal pathway and protect nerve cells.

关键词

Keywords

references

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温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3β表达的影响

Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment

DOI：10.13422/j.cnki.syfjx.20201304

摘要

Abstract

关键词

Keywords

references