Objective

2

Metabolomics was used to analyze the dynamic changes of endogenous metabolites in serum and lung tissue of rats treated with

water elution section of Siegesbeckiae Herba (SWES)

and to explore the possible mechanism of lung injury and to search for the sensitive markers in serum and lung tissue.

Method

2

SD rats were randomly divided into three groups

namely the normal group (normal saline)

SWES high-dose group and SWES low-dose group (0.500

0.125 g·kg

-1

·d

-1

). SWES was given for 4 weeks and stopped for 2 weeks. The weight and pathological examination were regarded as observation parameters. Serum and lung tissue samples were detected by nuclear magnetic resonance hydrogen spectrum (

1

H-NMR). The metabolites in rats were analyzed by partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA).

Result

2

Lung inflammation was shown in SWES high-dose group and returned to normal after withdrawal for 2 weeks. The metabolic spectrum of SWES high-dose group was significantly different from the normal group. There were 11 metabolites were identified by

1

H-NMR metabolomics

four differential metabolites were identified in serum [acetate

trimethylamine oxide (TMAO)

glycine

myo-inositol] and nine differential metabolites were identified in lung tissue [lactate

acetate

phosphatidylcholine (PC)

pyruvate

dimethylamine

glutamate

glycerophosphocholine (GPC)

glycine

xanthine]

. Lung injury was related to the disorder of pyruvate metabolism

glutamate metabolism and other pathways. Most of the metabolites in lung tissue had obviously levels of callback after drug withdrawal

which coincided with the pathological examination.

Conclusion

2

The lung is one of the damaged organs caused by SWES

and the lung injury is reversible and may be related to energy metabolism and oxidative stress.