Objective

2

To observe

Plantaginis Semen's mechanism in treating diarrhea by observing the effect on inflammatory factors in serum and mRNA and protein expressions of aquaporin4 (AQP4) in colon tissue of diarrhea rats.

Method

2

Senne Folium was orally administered to duplicate diarrhea rats. Sixty male SD rats were randomly divided into normal group

model group

hydrochlorothiazide group (9 mg·kg

-1

)

and low

middle

and high-dose Plantaginis Semen groups (0.95

1.9

3.8 g·kg

-1

). Senne Folium

(20 mL·kg

-1

) was intragastrically administered in 5 groups in the morning

except for normal group that was orally given the same dose of distilled water. In the afternoon

each treatment group was orally given the corresponding drugs

while normal group and model group were orally given the same dose of distilled water. The loose stool rate

average degree of loose stool

and diarrhea index were compared according to fecal traits and stool times after 14 days of treatment. The serum and colon tissue were collected to detect the contents of tumor necrosis factor-

α

(TNF-

α

)

interleukin-6 (IL-6) and C-reactive protein (CRP) in serum. Hematoxylin-eosin (HE) staining was used to observe the pathological morphological changes of colon tissue

and quantiative Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR) and Western blot were used to detect the mRNA and protein expressions of AQP4 in colon tissue.

Result

2

In the model group

the loose stool rate

average degree of loose stool

and diarrhea index were significantly increased (

P

<

0.01)

apoptosis and necrosis were observed in the epidermal cells of colonic mucosa

telangiectasia and congestion in lamina propria were obvious

and a few neutrophils were infiltrated

and the contents of TNF-

α

IL-6 and CRP in serum increased (

P

<

0.05

P

<

0.01)

the mRNA and protein expressions of AQP4 significantly decreased (

P<

0.01). Compared with the model group

the loose stool rate

average degree of loose stool

and diarrhea index were significantly decreased in low

middle

and high-dose Plantaginis Semen groups (

P

<

0.01)

the apoptosis and necrosis of epidermal cells

telangiectasia and hyperemia and neutrophil infiltration in colonic mucosa were obviously improved

and the contents of TNF-

α

and CRP in serum significantly decreased (

P

<

0.05

P

<

0.01)

the mRNA and protein expressions of AQP4 increased （

P<

0.05

P<

0.01）.

Conclusion

2

Plantaginis Semen has a better antidiarrheal effect

and its mechanism may be related to inhibition of inflammatory reaction

repair of pathological damage of colonic mucosa

up-regulation of AQP4 expression and promotion of water and fluid metabolism.