Objective

2

To study the effect of Qiwei Baizhusan (QWBZS) on liver insulin phosphatidylinositol 3-kinase（PI3K）/protein kinase B（Akt） signal pathway of diabetic mice induced by high-fat diet and streptozotocin (STZ).

Method

2

The methods of network pharmacology and animal experiments were used to study the hypoglycemic effect of QWBZS. Active chemical components of the drug and disease targets selected through public databases were used to construct the protein-protein interaction network (PPIN)

and gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomics(KEGG) enrichment analysis was performed to identify relevant signal pathways

in vivo

. In the pharmacological experiment

the diabetic mice model was established through intraperitoneal injection with 80 mg·kg

-1

·d

-1

STZ high-glucose

high-fat diet. The mice were divided into normal group (normal saline)

model group (normal saline) and QWBZS group (18.7 g·kg

-1

·d

-1

). After 28 days

the hypoglycemic effect of the drug and its effect on serum total cholesterol (T-CHO)

fasting insulin (FINS) and serum tumor necrosis factor-

α

(TNF-

α

) were determined. Western blot and Real-time fluorescence quantitative PCR (Real-time PCR) were used to detect protein and mRNA expressions of insulin receptor (IR)

insulin receptor substrate-1 (IRS-1)，phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt) in liver tissues．

Result

2

A total of 36 active components in this drug were identified by network pharmacology. KEGG analysis suggested that QWBZS might play a role in reducing blood glucose by regulating PI3K Akt signal pathway. Compared with the model group

the levels of blood glucose

serum T-Cho and TNF-

α

of the intervention group were significantly lower (

P

<

0.01)

while the FINS of the intervention group was significantly higher (

P

<

0.01). Protein and mRNA expressions of IR，IRS-1，PI3K and Akt in liver tissues of mice in QWBZS treatment group increased markedly (

P

<

0.05，

P

<

0.01).

Conclusion

2

QWBZS could regulate the levels of blood glucose

TNF-

α

T-CHO

and FINS in the serum of diabetic mice induced by high-fat diet and STZ. It can improve PI3K/Akt signal pathway of diabetic mice by regulating protein and mRNA expressions of IR，IRS-1，PI3K and Akt/PKB.