3首止泻名方治疗溃疡性结肠炎大鼠的相关机制对比分析

马旭冉; 王彦礼; 邹迪新; 王敦方; 宋红新; 杨伟鹏; 葛永祥

doi:10.13422/j.cnki.syfjx.20202004

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3首止泻名方治疗溃疡性结肠炎大鼠的相关机制对比分析

经典名方 | 更新时间：2020-09-16

- 3首止泻名方治疗溃疡性结肠炎大鼠的相关机制对比分析
- Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments
- 中国实验方剂学杂志 2020年26卷第20期页码：1-8
- 作者机构：
  
  1.中国中医科学院中药研究所，北京 100700
  2.北京市中西医结合医院，北京 100039
- 作者简介：
  
  马旭冉，博士，从事中药药理学研究，E-mail：maxuran194@163.com
  杨伟鹏，博士，研究员，从事中药药理学研究，E-mail：hrbywp@sina.com；
  葛永祥，硕士，副主任医师，从事中西医结合治疗胃肠病和肝病研究，E-mail：gyx19722007@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81473592;81273662);中央级公益性科研院所基本科研业务费专项(ZXKT17029;ZXKT18016)
- DOI：10.13422/j.cnki.syfjx.20202004
  中图分类号： R2-0;R22;R285.5;R289
- 收稿日期：2020-03-02，
  
  网络出版日期：2020-07-27，
  
  纸质出版日期：2020-10-20
- 稿件说明：
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马旭冉,王彦礼,邹迪新等.3首止泻名方治疗溃疡性结肠炎大鼠的相关机制对比分析[J].中国实验方剂学杂志,2020,26(20):1-8.

MA Xu-ran,WANG Yan-li,ZOU Di-xin,et al.Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):1-8.
马旭冉,王彦礼,邹迪新等.3首止泻名方治疗溃疡性结肠炎大鼠的相关机制对比分析[J].中国实验方剂学杂志,2020,26(20):1-8. DOI： 10.13422/j.cnki.syfjx.20202004.

MA Xu-ran,WANG Yan-li,ZOU Di-xin,et al.Comparison of Related Mechanism in Rats with Ulcerative Colitis After Treatment of Three Regiments[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):1-8. DOI： 10.13422/j.cnki.syfjx.20202004.

摘要

目的

对比研究黄芩汤、四神丸、痛泻要方3首止泻名方对大鼠溃疡性结肠炎(UC)炎症反应、水运调节及脑肠轴的影响。

方法

42只SPF级雄性SD大鼠，以2

6-三硝基苯磺酸(TNBS)复合造模法制备UC大鼠模型，按体质量随机分为正常组、模型组、阳性药柳氮磺胺吡啶组(SASP，0.5 g·kg

-1

)，黄芩汤组(Huangqintang，HQT，20 g·kg

-1

)，四神丸组(Sishen Wan，SSW，26 g·kg

-1

)和痛泻要方组(Tongxie Yaofang，TXYF，22 g·kg

-1

)。连续给药5 d后，大鼠眼眶取血并处死取结肠，苏木素-伊红（HE）染色检测大鼠结肠组织病变损伤程度，酶联免疫吸附测定(ELISA)结合免疫组化(IHC)测定大鼠血清及结肠组织内血管活性肠肽(VIP)，5-羟色胺(5-HT)，P物质(SP)分布，蛋白免疫印迹法(Western blot)检测大鼠结肠组织内水通道蛋白3(AQP3)，水通道蛋白4(AQP4)蛋白水平，实时荧光定量聚合酶链式反应(Real-time PCR)检测大鼠结肠组织内细胞外调节蛋白激酶1(Erk1)，p38丝裂原活化蛋白激酶(p38 MAPK) mRNA表达。

结果

与正常组比较，模型组5-HT，VIP含量均显著下降(

0.01)，SP含量降低，但未见显著性统计学差异；与模型组比较，SASP组和TXYF组5-HT含量明显升高(

0.05)，SASP组，HQT组，TXYF组VIP，SP含量均明显升高(

0.05)。与正常组比较，模型组AQP3含量显著升高(

0.01)，AQP4含量显著降低(

0.01)；与模型组比较，HQT组AQP3含量显著降低(

0.01)，SASP组，HQT组AQP4含量明显升高(

0.05)；与模型组比较，各给药组Erk1，p38 MAPK mRNA表达均显著降低(

0.01)，HQT组降低趋势最为显著。

结论

3首名方对大鼠溃疡性结肠炎均有一定治疗作用，可有效改善UC大鼠的体征及精神状况，但在改善泄泻状态下脑肠轴紊乱方面，TXYF治疗作用优于HQT和SSW，在抑制炎症反应及调节水运平衡方面，HQT治疗作用优于TXYF和SSW。

Abstract

Objective

This study was designed to compare inflammatory response

water carriage and gut brain axis in rats with ulcerative colitis (UC) after treatment of three regiments

Huangqintang (HQT)

Sishenwan (SSW)

and Tongxie Yaofang(TXYF).

Method

After approved by Institute of Chinese Materia Medica Ethics Committees in China Academy of Chinese Medical Sciences

UC in rats was induced by using a compound method (trinitrobenzenesulfonic acid plus ethanol). Rats were randomly divided into control

disease

positive control salazosulfapyridine (SASP

0.5 g·kg

-1

)

HQT (20 g·kg

-1

)

SSW(26 g·kg

-1

)

and TXYF group(22 g·kg

-1

). After 5 days of treatment

colonic tissues and the blood were taken for various assays. Damage of colonic tissues was detected by hematoxylin-eosin staining (HE). The distribution of Vasoactine intrestinal (VIP)

5-hydroxytrytamine (5-HT)

P-substance (SP) in the blood and serum were detected by enzymelinked immunosorbent assay (ELISA) and immunohistochemistry (IHC)

the levels of aquaporin3 (AQP3) and Aquaporin4 (AQP4) in the serum were detected by Western blot

the mRNA expression of Extracellular regulated protein kinases 1 (Erk1) and p38 mitogen-activated protein kinase (p38 MAPK) in the serum were detected by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR).

Result

The brain-gut peptide results showed that compared with the normal group

the content of 5-HT and VIP in model group were significantly decreased (

0.01)

the content of SP were decreased

but there was no significant statistical difference

compared with the disease group

the content of 5-HT in SASP and TXYF group were clearly increased (

0.05)

the increment of VIP and SP in SASP

HQT

TXYF group were significant (

0.05). Compared with the normal group

the content of AQP3 in model group were significantly increased(

0.01)

the content of AQP4 were clearly decreased(

0.01)

compared with the disease group

the content of AQP4 in SASP and HQT group were clearly increased (

0.05)

whereas the levels of AQP3 in HQT group were most significant reduced (

0.01). Compared with the disease group

the expression of Erk1 and p38 were clearly reduced (

0.01)

with the most significant reduce being the expression in HQT group.

Conclusion

Three regiments all have therapeutic effects on UC

manifested by improvements of the signs and mental status of UC rats. However

in terms of gut-brain axis disturbance improvement

the therapeutic effect of TXYF was superior than HQT and SSW

whereas in terms of inflammatory response suppression and water carriage accomodation

the therapeutic effect of HQT was superior than SSW and TXYF.

关键词

Keywords

references

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