泽漆醇提物对LPS诱导小鼠急性肺损伤及其炎症信号通路MAPK/NF-<italic style="font-style: italic">κ</italic>B的影响

陈兰英; 尹力; 周朦静; 邵峰; 石强; 刘荣华

doi:10.13422/j.cnki.syfjx.20202039

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泽漆醇提物对LPS诱导小鼠急性肺损伤及其炎症信号通路MAPK/NF-κB的影响

药理 | 更新时间：2020-09-16

- 泽漆醇提物对LPS诱导小鼠急性肺损伤及其炎症信号通路MAPK/NF-κB的影响
- Effect of Euphorbia helioscopia Alcohol Extract on MAPK/NF-κB Inflammation Pathway on Mice with Acute Lung Injury Induced by LPS
- 中国实验方剂学杂志 2020年26卷第20期页码：46-51
- 作者机构：
  
  1.江西中医药大学中药固体制剂制造技术国家工程研究中心，南昌 330006
  2.江西中医药大学药学院，南昌 330004
  3.江西中医药大学岐黄国医书院，南昌 0025
- 作者简介：
  
  陈兰英，博士，教授，从事中药药效评价与作用机制研究，E-mail：clyxy2513@163.com
  刘荣华，博士，教授，从事中药质量和化学研究，Tel：0791-87118992，E-mail：rhliu@163.com
- 基金信息：
  
  江西省重点研发计划项目(20181ACG70014);国家自然科学基金项目(81560637);江西省中药学一流学科专项科研基金项目(JXSYLXK-ZHYAO124)
- DOI：10.13422/j.cnki.syfjx.20202039
  中图分类号： R2-0;R289;R256.1
- 收稿日期：2020-05-30，
  
  网络出版日期：2020-08-14，
  
  纸质出版日期：2020-10-20
- 稿件说明：
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陈兰英,尹力,周朦静等.泽漆醇提物对LPS诱导小鼠急性肺损伤及其炎症信号通路MAPK/NF-κB的影响[J].中国实验方剂学杂志,2020,26(20):46-51.

CHEN Lan-ying,YIN Li,ZHOU Meng-jing,et al.Effect of Euphorbia helioscopia Alcohol Extract on MAPK/NF-κB Inflammation Pathway on Mice with Acute Lung Injury Induced by LPS[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):46-51.
陈兰英,尹力,周朦静等.泽漆醇提物对LPS诱导小鼠急性肺损伤及其炎症信号通路MAPK/NF-κB的影响[J].中国实验方剂学杂志,2020,26(20):46-51. DOI： 10.13422/j.cnki.syfjx.20202039.

CHEN Lan-ying,YIN Li,ZHOU Meng-jing,et al.Effect of Euphorbia helioscopia Alcohol Extract on MAPK/NF-κB Inflammation Pathway on Mice with Acute Lung Injury Induced by LPS[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):46-51. DOI： 10.13422/j.cnki.syfjx.20202039.

摘要

目的

研究泽漆醇提物对脂多糖（LPS）所致急性肺损伤小鼠的保护作用并探讨其可能的作用机制。

方法

50只雄性BALB/c小鼠按体质量随机分为正常组，模型组，地塞米松组（1.5 mg·kg

-1

）及泽漆醇提物高、低剂量组（7.5，3.75 g·kg

-1

）。除正常组外，其余各组采用鼻腔内滴入LPS建立小鼠急性肺损伤模型。利用全自动血液分析仪以及瑞氏-姬姆萨复合染色法检测并观察支气管肺泡灌洗液（BALF）中炎性细胞种类及数量；苏木素-伊红（HE）染色观察肺组织损伤情况；流式细胞仪检测BALF中炎性因子如肿瘤坏死因子-

（TNF-

），白细胞介素-6（IL-6）的含量；蛋白免疫印迹法（Western blot）测定核转录因子-

B（NF-

B）通路中NF-

B p65，NF-

B抑制蛋白

（I

），丝裂原活化蛋白激酶（MAPK）通路中c-Jun氨基末端激酶（JNK），p38，细胞外调节蛋白激酶（ERK1/2）及其磷酸化蛋白表达。

结果

与正常组比较，模型组小鼠肺组织出现明显损伤，其中大量炎性细胞浸润，且肺泡完整性被破坏。BALF中炎性因子TNF-

，IL-6及肺组织NF-

B p65，JNK，p38，ERK1/2磷酸化蛋白表达水平显著升高（

0.01）；与模型组比较，泽漆醇提物高剂量组及地塞米松阳性药组小鼠肺组织病理损伤明显缓解，BALF中TNF-

α，

IL-6含量及肺组织NF-

B p65，JNK，p38，ERK1/2磷酸化蛋白表达水平显著下调（

0.01）。

结论

泽漆醇提取物对LPS诱导小鼠急性肺损伤具有保护作用，其机制可能与调控MAPK/NF-

B信号通路有关。

Abstract

Objective

To study the protective effect of

Euphorbia helioscopia

alcohol extract on lipopolysaccharide (LPS) -induced acute lung injury in mice and explore its possible mechanism.

Method

The 50 Balb/c male mice were randomly divided into 5 groups

including normal group

model group

dexamethasone group (1.5 mg·kg

-1

)

E. helioscopia

alcohol extracts group (7.5，3.75 g·kg

-1

). Except for the normal group

the other groups used intranasal instillation of LPS to establish a model of acute lung injury in mice. The type and number of inflammatory cells in bronchoalveolar lavage fluid (BALF) were detected by automatic blood analyzer and Wright-Giemsa composite staining. The lung tissue damage was observed by hematoxylin-eosin (HE) staining. The contents of the inflammatory factors tumor necrosis factor-

(TNF-

) and interleukin-6 (IL-6) in BALF were detected by flow cytometry. The protein expressions of nuclear factor kappa-B p65(NF-

B p65)

phospho-NF-

B p65 (p-NF-

B p65)

inhibitor of NF-

)

phospho-I

(p-I

) in NF-

B pathway and c-Jun N-terminal kinase (JNK)

phospho-JNK (p-JNK)

p38 protein (p38)

phospho-p38 (p-p38)

extracellular regulated protein kinases (ERK1/2)

phospho-ERK1/2 (p-ERK1/2) in mitogen-activated protein kinase (MAPK) pathway were determined by Western blot.

Result

Compared with normal control group

the lung tissue of the model group showed obvious damage

in which a large number of inflammatory cells infiltrated

and the integrity of the alveoli was destroyed. Inflammatory factors TNF-

IL-6 in BALF and p-NF-

B p65

p-JNK

p-p38

p-ERK protein expression levels in lung tissue were significantly increased (

0.01). Compared with model group

the pathological damage of lung tissue in mice with high dose of

E. helioscopia

alcohol extract and dexamethasone positive group was significantly alleviated. The levels of TNF-

and IL-6 in BALF and the expression levels of p-NF-

B p65

p-JNK

p-p38 and p-ERK1/2 protein in lung tissue were significantly down-regulated (

0.01).

Conclusion

The

E. helioscopia

alcohol extract has a protective effect on LPS-induced acute lung injury in mice

its mechanism may be related to the regulation of the NF-

B/MAPK signaling pathway.

关键词

Keywords

references

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Effect of Euphorbia helioscopia Alcohol Extract on MAPK/NF-κB Inflammation Pathway on Mice with Acute Lung Injury Induced by LPS

DOI：10.13422/j.cnki.syfjx.20202039

摘要

Abstract

关键词

Keywords

references