Objective

2

To study the volatile oil of

Notopterygium inchum

and

N. franchetii

by using gas chromatography-mass spectrometry （GC-MS）.

Method

2

Ten batches of

N. inchum

and

N. franchetii

were harvested from Sichuan and Gansu，including eight batches of wild samples， one batch of cultivated

N. inchum

and one batch of cultivated

N. franchetii

. Volatile oil was extracted according to the methods in

Chinese Pharmacopeia

， and the chemical constituents were analyzed with GC-MS technology.

Result

2

Ninety-seven chemical compounds were identified，including monoterpenes，sesquiterpenes and non-terpenes. The calculation results of the relative content of chemical components showed that

α

-pinene（25.39%-41.68%），

β

-pinene（19.34%-40.95%） and limonene（4.59%-11.99%） were main compounds in volatile oil of

N. inchum

and

N. franchetii

. Relative content of

α

-pinene was higher than that of

β

-pinene in wild

N. inchum

and cultivated

N. franchetii

， while relative content of

β

-pinene was higher than that of

α

-pinene in wild

N. franchetii

. Relative content of

β

-pinene in samples stored for one year was lower than that of newly harvested ones. The partial least squares discriminant analysis with SIMCA-P+11.5 software showed that the samples from Gansu and Sichuan provinces had no significant differences， and additionally，one-year-stored samples and newly-harvested samples were clustered into two groups.

Conclusion

2

This study showed that the storage had influence on the chemical constituents of volatile oil in

N. inchum

， and it also revealed the differences in volatile oil components of wild and cultivated

Notopterygium

. The above mentioned work provided chemical basis for the pharmacology comparison study of volatile oil from different kinds of

N. inchum

and

N. franchetii.