Objective

2

To study the microbial community composition and diversity of brown-rot

Gastrodia elata

and its surface soil，in order to explain the relationship between brown-rot

G. elata

and soil microflora in

G. elata

planting process and provide theoretical basis for revealing the reasons of

G. elata

brown-rot disease.

Method

2

Used internal transcribed spacer region（ITS） and 16S rDNA high-throughput sequencing technologies to detect the microbial diversity，community structure composition and community structure similarity of fungi and bacteria in healthy tuber，Brown-rot tuber，healthy soil and Brown-rot soil.

Result

2

Compared with health groups，the number of fungi and bacteria operational taxonomic units（OTUs） was increased in brown-rot

G. elata

and its soil， and the abundance and diversity of fungi and bacteria in brown-rot

G. elata

soil were significantly decreased. The diversity of fungi in the tubers of brown-rot

G. elata

was significantly reduced，while the diversity of bacteria was significantly decreased. At the genus level，

Mortierella

was dominant fungi genus in healthy tuber and healthy soil，which was reduced 7.62% and 15.75%

respectively in brown-rot tuber and brown-rot soil. And the dominant bacteria genus was

Bradyrhizobium

and

Burkholderia-Paraburkholderia

respectively.

Ilyonectria

was dominant fungi genus in brown-rot tuber and brown-rot soil，the dominant bacteria genus was

Serratia

and

Bradyrhizobium

respectively.

Conclusion

2

The fungal flora in the tuber of brown-rot

G. elata

had a very high degree of similarity to that in the surrounding soil. These results indicated that the change of soil microbial fungal community caused the occurrence of

G. elata

brown-rot disease to a certain extent. And the pathogenic fungal

Ilyonectria

was dominant genus in fungi community of brown-rot tuber and brown-rot soil.

Ilyonectria

may have the main

G. elata

brown-rot disease pathogen.