Objective

2

The functional model of six major components of Astragali Radix-Angelicae Sinensis Radix combination against the proliferation of vascular smooth muscle cells （VSMCs） was constructed by uniform design， the relationship between the compatibility of these six main components and the inhibition of VSMCs proliferation was analyzed， and the effect of the compatibility of these main components of Astragali Radix-Angelicae Sinensis Radix on the proliferation of VSMCs as well as the feasibility of uniform design test in the study of multi-component compatibility of Chinese medicines were discussed.

Method

2

Cell proliferation and toxicity assay kit （CCK-8） method was used to determine the inhibitory effect of the six components of Astragali Radix-Angelicae Sinensis Radix on platelet derived growth factor-BB （PDGF-BB）-induced VSMCs proliferation in rats and the half inhibitory concentration （IC

50

） of each component were obtained. Six chemical components of Astragali Radix-Angelicae Sinensis Radix （formononetin， astragaloside Ⅰ， astragaloside Ⅳ， calycosin， ferulic acid and calycosin-7-

O

-

β

-

D

-glucoside） were taken as the independent variables

X

1

，

X

2

，

X

3

，

X

4

，

X

5

，

X

6

， respectively， and the cell proliferation inhibition rate as the dependent variable

Y

. U

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2.20133328

4.40266657

（24

9

） uniform design table and mathematical models （including linear and quadratic polynomial） were selected， the stepwise regression method was used to screen the variables， and the better equations were obtained in theory. The actually better relationship equations were screened by setting the different dose compatibility of these six components for verification tests. According to the purpose of the experiment， the selected regression equation was processed by data centralization， so as to compare the regression coefficients of the data centralization equation and analyze the correlation between components and efficacy.

Result

2

The six components of Astragali Radix-Angelicae Sinensis Radix could inhibit abnormal proliferation of VSMCs in a dose-dependent manner. IC

50

values of formononetin， astragaloside Ⅰ， astragaloside Ⅳ， calycosin， ferulic acid and calycosin-7-

O

-

β

-

D

-glucoside were 123.453， 113.184， 81.655， 141.159， 101.187， 151.016 mg·L

-1

， respectively. Two superior models were selected by experimental verification， including equation 1 of

Y

1

=73.137 897-0.207 888

X

1

+0.000 508

X

2

2

+0.000 347

X

1

X

6

and equation 2 of

Y

2

=70.038 433-0.236 665

X

1

+0.000 577

X

2

2

+0.000 260

X

1

X

4

+0.000 415

X

1

X

6

+0.000 370

X

3

X

5

. After data centralization， equation 2 was adjusted to

Y

=-1.480 260-0.146 281

X

1

+0.000 673

X

2

2

+0.000 314

X

1

X

4

+0.000 456

X

1

X

6

+0.000 737

X

3

X

5

， the regression coefficients showed that the astragaloside Ⅰ had a positive effect on the inhibition rate of cell proliferation， formononetin-calycosin， formononetin-calycosin-7-

O

-

β

-

D

-glucoside and astragaloside Ⅳ-ferulic acid had an interaction effect on the inhibition rate of cell proliferation， and the synergistic effect of astragaloside Ⅳ and ferulic acid was higher than other interaction items.

Conclusion

2

The combination of six main components of Astragali Radix-Angelicae Sinensis Radix can inhibit the abnormal proliferation of VSMCs， and it is demonstrated that the uniform test design can be used to predict the correlation between the chemical composition of Chinese medicines with their pharmacological effects， which can provide an experimental basis for the rational use of uniform design and regression analysis to construct mathematical models to study the compatibility relationship of Chinese medicines.