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北京中医药大学 第三附属医院,北京 100029
林陶秀,在读硕士,从事中医药治疗子宫内膜异位症的研究,E-mail:lintaoxiu@126.com
马小娜,博士,教授,主任医师,博士生导师,从事中医药治疗子宫内膜异位症的研究,E-mail:fightandfight@yeah.net
收稿日期:2022-04-27,
网络出版日期:2022-07-20,
纸质出版日期:2023-01-20
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林陶秀,张文娟,张悦健等.最佳中药含药血清浓度的筛选方法研究现状[J].中国实验方剂学杂志,2023,29(02):195-202.
LIN Taoxiu,ZHANG Wenjuan,ZHANG Yuejian,et al.Screening Methods for Optimal Serum Concentration of Chinese Medicine: A Review[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(02):195-202.
林陶秀,张文娟,张悦健等.最佳中药含药血清浓度的筛选方法研究现状[J].中国实验方剂学杂志,2023,29(02):195-202. DOI: 10.13422/j.cnki.syfjx.20221505.
LIN Taoxiu,ZHANG Wenjuan,ZHANG Yuejian,et al.Screening Methods for Optimal Serum Concentration of Chinese Medicine: A Review[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(02):195-202. DOI: 10.13422/j.cnki.syfjx.20221505.
基于血清药理学为理论基础的体外细胞实验是研究中药药效机制的一种常见的研究方式,实验过程中筛选中药含药血清最佳干预浓度是整个实验的关键步骤。中药含药血清若稀释太过,或浓度太大,均可能导致出现假阴性或假阳性结果,最佳中药含药血清体积分数也可以减少后续实验分组数量,减少操作失误,因此筛选最佳干预血清体积分数对于研究中药药效机制的体外细胞实验具有重要意义。但筛选方法目前尚无统一的标准,查阅近20年国内外文献,从中药灌胃剂量、采血时间、血清稀释程度3个方面进行整理及分析,进而总结出最佳含药血清体积分数的筛选方法,为中药药效机理研究提供指导方向。筛选方法主要有“同剂量灌胃,同采血时间,不同体积分数” “不同剂量灌胃,同采血时间,同体积分数” “同剂量灌胃,不同采血时间,同体积分数” “不同剂量灌胃,不同采血时间,不同体积分数” “血清冻干粉干预法” “血清模拟西药体积分数梯度法”“纯血清干预法”。前2种筛选方法是目前相关实验研究较为常用的方式,以上筛选方法各有利弊,研究者应该根据实验的具体需求及实际情况进行合理选择。
In vitro
cell experiment based on serum pharmacology is a common way to study the pharmacodynamic mechanism of Chinese medicine, and screening the optimal intervention concentration of serum containing Chinese medicine is a key step in the whole experiment. Over-diluted serum containing Chinese medicine or over-high concentration leads to false negative or positive results, while the optimal concentration of medicated serum reduces the number of groups in subsequent experiments as well as the operation error. Thus it is of great significance to screen the optimal serum concentration in studying the pharmacodynamic mechanism of Chinese medicine in
in vitro
cell experiments. However, there has been no unified standards for the screening methods at present. After reviewing the literature in China and abroad in the past 20 years, this paper conducted an analysis from three aspects of intragastric dose of Chinese medicine, blood collection time and serum dilution degree, and then summarized the screening methods for optimal concentration of serum containing drugs, providing guidance for the study of pharmacodynamic mechanism of Chinese medicine. The screening methods mainly included "same intragastric dose, same blood collection time, and different concentrations" "different intragastric doses, same blood collection time, and same concentration" "same intragastric dose, different blood collection time, and same concentration" "different intragastric doses, different blood collection time, and different concentrations" "serum lyophilized powder" "simulation of gradient concentration of serum containing western medicine" and "pure serum intervention". Among them, the former two were the dominant ways in current related research. The above screening methods had their own advantages and disadvantages, and researchers should make a reasonable choice according to the specific requirements and actual situation of the experiments.
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