Objective: To innovate and optimize the extract process of volatile oil in Tongjingbao granules. Method: Ligustilide

cinnamaldehyde

costunolide and dehydro-α-curcumene were used as chemical index and together with mice dysmenorrheal model and rat uterine spasm model

the best extract process of volatile oil in Radix Angelicae Sinensis

Cortex Cinnamomi Cassiae and Radix Aucklandiae were optimized. It was investigated by orthogonal experiment to optimize the conditions and parameters to extract essential oil. Result: Both the best extract process of Radix Angelicae Sinensis (single)

Cortex Cinnamomi Cassiae and Radix Aucklandia (mix) were CO2 supercritical fluid extraction

and the spasmolysis effect of innovated process had significant increase than the original preparation. The optimal extraction conditions of the essential oil from Angelica sinensis using supercritical fluid extraction were: pressure 25 MPa

temperature 40 ℃

extraction time 2 hour

and 35 MPa

55 ℃ and 2 hour for essential oil from Cinnamomum cassia and Aucklandia lappa. Conclusion: The improvement of the extraction process of volatile oil enhanced the efficacy of Tongjingbao preparation

and the process was feasible and convenient.