Objective: To discuss the effects of different drying methods of Qushi Huayu decoction on the pharmacodynamics of experimental fatty liver. Method: Qushi Huayu decoction was prepared(0.93 g crude drug ·mL-1)

then been concentrated to paste

and dried powder through 70 ℃ vacuum drying

40 ℃ vacuum drying

frozen drying and spray drying. Wistar male rats were used to establish fatty liver model by carbon tetrachloride (CCl4) subcutaneous injection for 4 weeks (twice per week) along with high-fat and low-protein diet for 2 weeks. After 2 weeks

the rats were respectively divided into model group

non-drying group

paste group

70 ℃ vacuum drying group

40 ℃ vacuum drying group

frozen drying group and spray drying group randomly and given corresponding drug. The rats were killed after treatment for 2 weeks

then activity of serum ALT

AST

GGT

and content of liver TG

FFA were detected. The different drugs were analyzed through fingerprint analysis. Result: Activity of serum ALT

AST

GGT and content of liver TG and FFA were all increased in the model group. Compared with the model group

ALT

AST

GGT

TG and FFA of the non-drying group were significantly decreased.The paste group had good effect on ALT

GGT

TG and FFA.Compared with the model group

activity of serum ALT were significantly decreased in 70 ℃ vacuum drying and 40 ℃ vacuum drying group

content of liver TG were significantly decreased in frozen drying group

activity of serum GGT were significantly decreased in spray drying group. Fingerprint Analysis showed the chromatogram map of each drug were different. Conclusion: Different drying methods have negative effects for Qushi Huayu decoction on the pharmacodynamics of experimental fatty liver. Some effective material may be dropped after drying. It needs further research.