Objective: Analysis the difference of urine metabolites between hypertension patients with the syndrome of ascendant hyperactivity of liver yang and healthy volunteers

search the possible biomarker

reveal the nature of the syndrome of ascendant hyperactivity of liver yang. Method: According to the clinical diagnostic criteria of the syndrome of ascendant hyperactivity of liver yang

select typical cases

select nine typical cases and eleven healthy volunteers

analysis the components in the urine derivatives of hypertension patients with the syndrome of ascendant hyperactivity of liver yang and healthy volunteers by gas chromatography mass spectrometry

match and align the data

select the same features

make the PCA and PLS-DA by the software Simca-P

track biomarkers and explain the possible relevant metabolic pathways. Result: The data from the analysis by PCA and PLS-DA of the urine samples shows that:two sets of data from GC-MS can be classified in the score plot.Some significantly changed metabolites like stearic acid

inositol

D-fructose

phthalic acid have been identified between the two groups in terms of relative metabolism of body. Conclusion: The metabonomics method

gas chromatography-mass spectrometry combined with pattern recognition

has advantages in studying pathophysiological changes of the body under the complicated conditions.The study provided a scientific basis for the the essence of traditional chinese medicine syndrome theory and clinical diagnosis of the syndrome of ascendant hyperactivity of liver yang.