Objective: To observe the change of the growth and mTOR signal transduction pathway after treatment with inhibitor in human endometrial carcinoma cell ishikawa in vitro.To provide an experimental foundation of the research on the pathogenesis of human endometrial carcinomas. Method: MTT and Clone formation test were used to determine the proliferative effects of two inhibitors on tumor cells.The mRNA levels of mTOR

the downstream phosporylated substrate S6K1 and STAT3 protein were assayed by RT-PCR and Western blot

respectively. Result: Rapamycin alone or combined with RG-14260 could inhibite cell proliferation and lower survival rate of ishikawa cells.The effect of rapamycin combinated with RG-14260was better than that of RG-14260 and rapamycin alone.There were high expression of mTOR mRNA and downstream phosporylated substrate S6K1 and STAT3 protein in ishikawa cells.But these index above were decreased after treatment with inhibitor. The effect of Rapamycin combinated with RG-14260 was more obvious than that of Rapamycin alone. Conclusion: mTOR signal transduction pathway play an important role in the formation and progress of endometrial carcinoma cells.