Objective: To study the quality standard of Aconiti Lateralis Radix Praeparata slice. Method : According to Chinese Pharmacopoeia

the total ash and acid insoluble ash were determined in Radix Aconiti Lateralis Praeparata slice. The determination of diester and monoester aconitum alkaloids was carried out by RP-HPLC method. HPLC analysis was performed on an Eclipse XDB-C18 (4.6 mm×250 mm

5 μm) column. The mobile phase system was 0.1 mol ·L-1 ammonium acetate (0.5 mL glacial acetic acid per 1 000 mL

phase A) and acetonitrile-tetrahydrofuran (25 ∶15

phase B) with gradient elution. The detection wavelength was at 235 nm. Result : There were obvious differences in content of total ash and acid-insoluble ash. The range of monoester aconitum alkaloids amounts was relatively narrow

but the range of diester aconitum alkaloids amounts was wider. It suggested that the range of diester aconitum alkaloids amounts should be formulated. Conclusion : The method is accurate

reproducible and can be used to enhance the quality control of Aconiti Lateralis Radix Praeparata slice.