Objective: To investigate the effect and mechanism of Xuefu Zhuyu Decoction inducing endothelial cells migration. Method: Serum pharmacology technique was adopted. Endothelial cells ECV304 were incubated with blank serum or Xuefu Zhuyu Decoction containing serum (XFZYD-CS) at final concentrations of 1.25%

2.5% and 5%. Cell migration was assessed by wound healing assay. Vascular endothelial growth factor(VEGF)

basic fibroblast growth factor(bFGF) and nitric oxide(NO) level in the culture supernate were evaluated by ELISA and nitrate reductase method respectively; intracellular NO was studied by fluorescent probe; endothelial nitric oxide synthase (eNOS) activity was assessed and VEGF

bFGF and eNOS expression were evaluated by RT-PCR. Result: 1.25%XFZYD-CS could induce cell migration in the wound healing model

5%XFZYD-CS could induce cell migration in Transwell

and 2.5%XFZYD-CS could work both methods. All 3 concentrations of XFZYD-CS could elevate bFGF

NO level in the culture supernate

intracellular NO level and eNOS activity. Only 2.5%XFZYD-CS could elevate supernate VEGF level. RT-PCR results showed that all 3 concentrations of XFZYD-CS could upregulate bFGF expression; 2.5% and 5%XFZYD-CS upregulated eNOS transcription; only 2.5%XFZYD-CS elevated VEGF expression

but 1.25%XFZYD-CS was opposite work. Conclusion: Xuefu Zhuyu Decoction could induce endothelial cell migration into angiogenesis via upregulating VEGF

bFGF and eNOS/NO pathways.