Objective: To investigate the therapeutical effect of a Chinese prescription with the function of reinforcing kidney and activating blood on immune premature ovarian failure (POF) in mice. Method: Each female BALB/c mouse was received multipoint immunization of porcine ZP4 (pZP4) in two hind footpads and intra-cutaneously to made the POF model. The mice were randomly divided into three Chinese medicine groups

contorl group

model group and estradiol (E2) group. After administrating Chinese medicine in three different doses (15.0

7.5

3.75 g·kg-1)

0.5% CMC-Na and E2 were given respectively for 20 days

vaginal smearses were visualized microscopically. E2 and pZP4 antibody levels were monitored. The morphology of ovarian tissue inflammation were studied. Result: Compared to the control group

the sex cycles of the high dose group was between 6 and 7 days without apparent change. Compared to the model group

the high dose group developed significantly higher serum E2 level (P<0.01) and lower serum antiboides to pZP4 (P<0.05)

and moreover

the number of primary follicles and secondary follicles were increased significantly (P<0.05). Conclusion: The Chinese prescription can improve the ovarian reproductive endocrine function and regulate the developmental capacity of ovarian follicle and corpus luteum in the four cycles. Otherwise

the Chinese medicine might inhibit the autoimmune presenility POF to some extent.