Objective: To study the active ingredients from Erzhi Pill(AIEP) on acute hepatic injury induced by carbon tetrachloride(CCl4)in mice. Method: Sixty Kunming mice were randomly divided into six groups: the normal group

the model group

bifendate group (150 mg·kg-1)

high AIEP group (19.8 g·kg-1)

middle AIEP group (13.2 g·kg-1)and low AIEP group (6.6 g·kg-1).The treatment groups were orally administered once per day for 7 d

whereas the normal and model groups were orally administered with saline. Except normal mice

all the other mice were injected intraperitoneally CCl4 20 mL·kg-1 once. The mice were executed 16 h after CCl4 administration. Serum and liver samples were collected for analysis. The acute hepatic injury model was prepared by CCl4 injected intraperitoneally. Then

the therapeutic effects of AIEP on the model were evaluated by the activity determination of serum alanine aminotransferase and aspirate aminotransferase (ALT and AST)

superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver

and the hepatic pathological changes following the treatment. Result: The activity of ALT and AST and the MDA content in liver were significantly increased and the activity of SOD was largely inhibited in the animals of modeling group. Following the treatment with AIEP

ALT and AST activity and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treatment group. Furthermore

AIEP could ameliorate the hepatic pathological changes. Conclusion: AIEP have protective effects on acute hepatic injury induced by CCl4 in mice.