Objective: To study the chemical constituents of Tongmai formula and set an example for its bioactive substances foundation investigation. Method: The chemical constituents were isolated and purified by column chromatography of macroporous resin

silica gel

Sephadex LH-20 and reversed phase high-performance liquid chromatography

and their structures were identified by physico-chemical properties and spectrum analysis. Result: Thirty-four compounds were separated and identified as tanshinone I (1)

cryptotanshinone (2)

dihydrotanshinone I (3)

formononetin (4)

protocatechuic aldehyde (5)

daidzein (6)

trans-ferulic acid (7)

ononin (8)

5-hydroxylononin (9)

senkyunolide H (10)

(4R)-3- -4-(4-β-D-glucopyranosyloxybenzyl)-but-2-en-4-olide (pueroside D

11)

genistin (12)

daidzin (13)

3'-methoxypuerarin (14)

formononetin-8-C-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (15)

formononetin-7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (16)

lanceolarin (17)

kakkanin (18)

biochanin A-8-C-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (19)

puerarin (20)

sucrose (21)

adenosine (22)

mirificin (23)

3'-hydroxypuerarin (24)

3'-methoxydaidzein-7

4'-di-O-β-D-glucopyranoside (25)

daidzein-7

4'-O-glucoside (26)

3'-methoxydaidzin (27)

formononetin-8-C-β-D-xylopyranosyl-(1→6)-O-β-D-glucopyranoside (28)

genistein-7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (ambocin

29)

genistein-8-C-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (30)

daidzein-7-O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranoside(31)

daidzein-7-O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranoside(32)

3'-hydroxymirificin (33)

6″-O-D-xylosylpuerarin (34). Conclusion: On the basis of the above mentioned results and previously reported chemical studies on the Puerariae Lobatae Radix

Salviae Miltiorrhizae Radix et Rhizoma

and Chuanxiong Rhizoma

it was suggested that the main chemical components were isoflavanoid compounds rised from the Puerariae Lobatae Radix

and the secondary components were tanshinones rised from the Salviae Miltiorrhizae Radix et Rhizoma

and senkyunolide H rised from the Chuanxiong Rhizoma in the Tongmai formula. Compaunds 16

17

29 were isolated from Puerariae Lobatae Radix and the genus Pueraria DC. for the first time.