Objective: To study liver and kidney injury induced by Tween 80

solutol HS 15 or polyoxyethylene (40) hydrogenated castor oil in rat. Method: The rats were randomly divided into such groups as control group

three dose groups of tween 80

three dose groups of solutol HS 15

three dose groups of polyoxyethylene (40) hydrogenated castor oil. Each rat was injected 0.22 mL of corresponding excipients q.d.for 4 weeks. Serum biochemical indicators of rat liver

kidney function were detected. Weight of liver

kidney was weighed and organ coefficient was calculated. Liver and kidney pathological changes were observed by HE staining. Result: There was no significant difference between groups of Tween 80 and the control group. There was significant difference in liver and kidney function in different dose groups of solutol HS15 and difference dose groups of polyoxyethylene (40) hydrogenated castor oil (P<0.05). There was no obvious pathological changes except some inflammatory cells in rat liver and kidney in rats in all the groups. Conclusion: Tween 80 might be the weakest one to cause liver and kidney injury in rats injected with the three kinds of excipients.