Objective: To study the effect of Lichong decoction on p53 signaling pathway gene expression profile in human uterine leiomyoma cells in vitro. Method: The primary cultured uterine leiomyoma cells were treated with Lichong decoction. Using PCR p53 signaling pathway microarray

all RNA was gained and cleanuped from human uterine leiomyoma cells in blank control group and Lichong decoction group. RNA yield and quality were assessed by UV absorbance and denaturing agarose gel electrophoresis

first strand cDNA Synthesis

performing Real-Time PCR finally. Result: Lichong decoction group was compared with blank control group among 96 pieces genes in PCR p53 signaling pathway microarray

17 in total(17.7%)genes expression had obviously changed

10 pieces went down obviously(Fold Down-Regulation2). The genes were generally classified

according to their functions

into apoptosis

cell cycle

cell growth

proliferation and differentiation and DNA repair. Conclusion: Lichong decoction can regulate correlation genes of apoptosis

cell cycle

cell growth

proliferation and differentiation and DNA repair in p53 signaling pathway of uterine leiomyoma

whichshow that signal-transmit between in and out of cell is the key link in uterine leiomyoma process

Lichong decoction has the effect of regulation and control of p53 signaling pathway gene expression profile.